Human His6Interleukin-25/IL-17E (hHis6IL-25)

• 产品编号：CST-12337LC      品牌：CST       原厂货号：12337LC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > IL-17 Family 白细胞介素-17超家族
• 应用分类：

描述：

重组人_His6IL-25 (h_His6IL-25) Tyr33-Gly177 (Accession #NP_073626) 通过Cell Signaling Technology公司的人源293细胞表达。重组的hIL-25蛋白在N-末端有His6标签，其分子量据推算为19,318 Da。DTT还原蛋白迁移率接近26-32kDa 的多肽。未还原的胱氨酸相连的同源二聚体迁移蛋白大约40-46 kDa。其在SDS-PAGE胶中的低迁移性和多态性与糖基化有关。重组h_His6IL-6蛋白的N-末端序列通过测序得到。通过SDS-PAGE确定还原型(+)和未还原型(-)的重组h_His6IL-25的纯度大于97%。在未还原的环境下，30%会作为单体发生迁移。所有样品的纯度均高于97%。

有载体：每微克h_His6IL-25溶解在包含 20 μg BSA和150 mM NaCl的20 mM Tris （pH 7.2）溶液中，并通过 0.22 μm 滤膜过滤后冻干。

无载体：每微克h_His6IL-25溶解在包含 150 mM NaCl的20 mM Tris （pH 7.2）溶液中，并通过 0.22 μm 滤膜过滤后冻干。L-25，又称IL-17E，是IL-17超家族细胞因子成员之一。IL-25在上皮细胞，CD4⁺T细胞，肥大细胞以及嗜酸性粒细胞中表达(1)。多种细胞类型都可以对IL-25产生应答，如T细胞，巨噬细胞和上皮细胞(1)。IL-25受体是由IL-17RA和IL-17RB组成的异源二聚体(1,2)。IL-25能够通过诱导IL-5、IL-4和IL-13促进Th2型免疫应答，同时可能有助于过敏性炎症和哮喘的发生(1-3)。IL-25同样能够促进Th9细胞的活化以及诱导乳腺癌细胞的凋亡(4,5)。

原厂资料：

Source / Purification

Recombinant human His6IL-25 (hHis6IL-25) Tyr33-Gly177 (Accession #NP_073626) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant N-terminally His6-tagged hIL-25 has a calculated MW of 19,318. DTT-reduced protein migrates as a 26-32 kDa polypeptide. The nonreduced cystine-linked homodimer migrates as a 40-46 kDa protein. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino terminus of recombinant hHis6IL-25 was verified by amino acid sequencing.

Purity

>97% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hHis6IL-25. 30% migrates as monomer under nonreducing conditions. All lots are greater than 97% pure.

Bioactivity

The bioactivity of recombinant hHis6IL-25 was determined by its ability to induce IL-5 production from TSLP-primed PBMC in the presence of IL-2. The ED50 of each lot is between 5-15 pg/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hHis6IL-25.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of hHis6IL-25 in 20 mM Tris, pH 7.2 containing 150 mM NaCl and 20 μg BSA per 1 μg hHis6IL-25. Carrier free: Lyophilized from a 0.22 μm filtered solution of hHis6IL-25 in 20 mM Tris, pH 7.2 containing 150 mM NaCl.

Background

IL-25, also known as IL-17E, is a member of the IL-17 superfamily of cytokines. IL-25 is expressed in epithelial cells, CD4+ T cells, mast cells, and eosinophils (1). Many cell types are responsive to IL-25, including T cells, macrophages, and epithelial cells (1). The receptor for IL-25 consists of a heterodimer of IL-17RA and IL-17RB (1,2). IL-25 promotes Th2 type immune responses by induction of IL-5, IL-4, and IL-13 and may contribute to allergic inflammation and asthma (1-3). IL-25 has also been shown in research to promote Th9 cell activation and induces apoptosis in breast cancer cells (4,5).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.