CDCP1 (D8M5K) Rabbit mAb

• 产品编号：CST-12327S      品牌：CST       原厂货号：12327S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CDCP1 (D8M5K) Rabbit mAb用于检测内源性的CDCP1总蛋白。该单克隆抗体可以识别CDCP1细胞外结构域的一个表位。该单克隆抗体由CDCP1蛋白Ala354附近残基合成的多肽免疫动物产生。CDCP1(CUB结构域包含蛋白, SIMA135)可能是一种干细胞的标记物，于多种研究中被发现在人癌细胞、典型和非典型结肠癌中高度表达（1）。CDCP1的表达可能受到表观遗传学调节，因为启动子CpG序列的甲基化可以导致CDCP1表达减少（2）。CDCP1的基因也编码由多种结构域形成的跨膜糖蛋白复合物抗原。3种胞外区的CUB结构域有可能在细胞粘附或细胞外基质相互作用中起作用（1,3）。Src-家族激酶可能磷酸化CDCP1胞浆区第5位的酪氨酸残基从而为包含SH2结构域的蛋白提供结合位点（3）。CDCP1可能是一种造血干细胞标记物(4,5)。

1. Scherl-Mostageer, M. et al. (2001) Oncogene 20, 4402-8.
2. Ikeda, J.I. et al. (2006) J. Pathol. 210, 75-84.
3. Hooper, J.D. et al. (2003) Oncogene 22, 1783-94.
4. Conze, T. et al. (2003) Ann. N.Y. Acad. Sci. 996, 222-226.
5. Bühring, H.J. et al. (2004) Stem Cells 22, 334-343.

原厂资料：

Specificity / Sensitivity

CDCP1 (D8M5K) Rabbit mAb recognizes endogenous levels of total CDCP1 protein. This antibody recognizes an epitope in the extracellular domain of CDCP1.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala354 of human CDCP1 protein.

Background

CDCP1 (CUB domain containing protein, SIMA135) is a putative stem cell marker that has been shown in research studies to be highly expressed in some human cancer cells and in both typical and atypical (cancerous) colons (1). Expression of CDCP1 may be epigenetically regulated, as methylation of promoter CpG sequences results in decreased CDCP1 expression (2). The corresponding CDCP1 gene encodes a glycoprotein that acts as a complex, multidomain transmembrane antigen. Three CUB domains in its extracellular region are possibly involved in cell adhesion or extracellular matrix interaction (1,3). Src-family kinases may phosphorylate CDCP1 at five tyrosine residues within its cytoplasmic domain to provide a potential binding site for SH2 domain-containing proteins (3). CDCP1 is a putative marker for hematopoietic stem cells (4,5).

1. Scherl-Mostageer, M. et al. (2001) Oncogene 20, 4402-8.
2. Ikeda, J.I. et al. (2006) J. Pathol. 210, 75-84.
3. Hooper, J.D. et al. (2003) Oncogene 22, 1783-94.
4. Conze, T. et al. (2003) Ann. N.Y. Acad. Sci. 996, 222-226.
5. Bühring, H.J. et al. (2004) Stem Cells 22, 334-343.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.