c-Myb (D2R4Y) Rabbit mAb

• 产品编号：CST-12319S      品牌：CST       原厂货号：12319S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

c-Myb (D2R4Y)Rabbit mAb可以识别内源性的总c-Myb蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人c-Myb蛋白的氨基末端邻近氨基酸残基序列一致。c-Myb是一个特异性识别5'-YAAC[GT]G-3'的转录激活因子。在造血祖细胞中表达，在控制增殖和分化过程中发挥了重要作用（1-3）。c-Myb对于小肠干细胞的自我更新中的基因转录是必须的。重要的是，c-Myb调控了Lgr5的表达，Lgr5表达在潜在的小肠干细胞中会形成小肠隐窝中的各种细胞系（4）。已经报道c-Myb在小肠隐窝细胞和人结肠直肠癌细胞中都有表达（5,6）。研究报道了多种白血病，乳腺癌和其他实体瘤中c-Myb基因发生转位和拷贝数变异（7,8）。

1. Lin, H.H. et al. (1996) Curr Top Microbiol Immunol 211, 79-87.
2. Mucenski, M.L. et al. (1991) Cell 65, 677-89.
3. Badiani, P. et al. (1994) Genes Dev 8, 770-82.
4. Cheasley, D. et al. (2011) Stem Cells 29, 2042-50.
5. Thompson, M.A. et al. (1998) Cancer Res 58, 5168-75.
6. Wilkins, H.R. et al. (2010) Tumour Biol 31, 16-22.
7. Ramsay, R.G. and Gonda, T.J. (2008) Nat Rev Cancer 8, 523-34.
8. Stenman, G. et al. (2010) Cell Cycle 9, 2986-95.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse

Specificity / Sensitivity

c-Myb (D2R4Y) Rabbit mAb recognizes endogenous levels of total c-Myb protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human c-Myb protein.

Background

c-Myb is a transcriptional activator that specifically recognizes the sequence 5'-YAAC[GT]G-3'. It is expressed in hematopoietic progenitor cells where it plays an important role in the control of proliferation and differentiation (1-3). c-Myb is required for transcription of genes involved in self-renewal of intestinal stem cells. Importantly, c-Myb regulates expression of Lgr5, a protein expressed in putative intestinal stem cells that give rise to all cell lineages of small intestinal crypts (4). c-Myb is reported to be expressed in colon crypt cells and in human colorectal cancer lines (5,6). Research has shown that c-Myb gene translocations and copy number alterations are found in several leukemias, breast cancer, and other solid tumors (7,8).

1. Lin, H.H. et al. (1996) Curr Top Microbiol Immunol 211, 79-87.
2. Mucenski, M.L. et al. (1991) Cell 65, 677-89.
3. Badiani, P. et al. (1994) Genes Dev 8, 770-82.
4. Cheasley, D. et al. (2011) Stem Cells 29, 2042-50.
5. Thompson, M.A. et al. (1998) Cancer Res 58, 5168-75.
6. Wilkins, H.R. et al. (2010) Tumour Biol 31, 16-22.
7. Ramsay, R.G. and Gonda, T.J. (2008) Nat Rev Cancer 8, 523-34.
8. Stenman, G. et al. (2010) Cell Cycle 9, 2986-95.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.