Phospho-Scribble (Ser1220) (D8A2) Rabbit mAb

• 产品编号：CST-12316S      品牌：CST       原厂货号：12316S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Scribble (Ser1220) (D8A2) Rabbit mAb可以识别Ser1220被磷酸化的内源性scribble蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人scribble蛋白Ser1220 附近氨基酸一致。Scribble(Scrib)最初是在果蝇中进行基因扫描时，和细胞极性决定蛋白Discs Large (Dlg) 和Lethal giant larvae (Lgl)一起发现的。将这些基因在果蝇体内进行纯和敲除会显示相似的表型，包括apicobasal细胞极性丢失以及肿瘤组织过度生长。这些相似的表型表示这三种蛋白在一个共同的通路中发挥重要作用以建立和维持表皮细胞中apicobasal的极性(1,2)。Scribble含有多个富含亮氨酸的重复以及PDZ结构域以将scribble定位到哺乳动物上皮细胞的黏附连接和基地外侧区域(3)。据报道，Scribble通过一个短基序和β-catenin, APC, E-cadherin 和高危型HPV病毒的E6蛋白结合，这种结合对E6蛋白诱导的细胞转化非常重要(4-8)。过表达scribble会通过HPV的E6/7蛋白抑制啮齿类上皮细胞转化(8).Scribble的磷酸化状态有重要功能，部分是通过调控亚细胞定位发挥的(9)。质谱分析显示Ser1220的磷酸化在多种细胞和组织类型中都是一个常见的修饰(10-13)。这个修饰的功能还有待阐明。

1. Bilder, D. and Perrimon, N. (2000) Nature 403, 676-80.
2. Bilder, D. et al. (2000) Science 289, 113-6.
3. Humbert, P.O. et al. (2008) Oncogene 27, 6888-907.
4. Sun, Y. et al. (2009) Mol Biol Cell 20, 3390-400.
5. Qin, Y. et al. (2005) J Cell Biol 171, 1061-71.
6. Navarro, C. et al. (2005) Oncogene 24, 4330-9.
7. Takizawa, S. et al. (2006) Genes Cells 11, 453-64.
8. Nguyen, M.L. et al. (2003) J Virol 77, 6957-64.
9. Yoshihara, K. et al. (2011) Exp Cell Res 317, 413-22.
10. Olsen, J.V. et al. (2010) Sci Signal 3, ra3.
11. Han, G. et al. (2010) Electrophoresis 31, 1080-9.
12. Brill, L.M. et al. (2009) Cell Stem Cell 5, 204-13.
13. Wang, Y.T. et al. (2010) J Proteome Res 9, 5582-97.

原厂资料：

Specificity / Sensitivity

Phospho-Scribble (Ser1220) (D8A2) Rabbit mAb recognizes endogenous levels of scribble protein only when phosphorylated at Ser1220.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1220 of human scribble protein.

Background

Scribble (Scrib) was originally identified in a genetic screen in Drosophila along with cell polarity determinants Discs Large (Dlg) and Lethal giant larvae (Lgl). Drosophila mutants homozygous for these genes share similar phenotypes, including the loss of apicobasal cell polarity and neoplastic tissue overgrowth. These phenotypic similarities suggest that these three proteins function in a common pathway important for establishing and maintaining apicobasal polarity in epithelial cells (1,2). Scribble contains many leucine-rich repeats and PDZ domains important for localizing scribble to adherens junctions and basolateral regions of mammalian epithelial cells (3). Scribble reportedly binds β-catenin, APC, E-cadherin and the E6 protein from high-risk virus type of HPV through a short motif important for E6-induced cell transformation (4-8). Overexpression of scribble inhibits transformation of rodent epithelial cells by HPV E6/7 proteins (8).The phosphorylation state of Scribble has been shown to be functionally important, in part by regulating subcellular localization (9). Mass spectrometry studies have identified phosphorylation at Ser1220 as a frequent modification in a variety of cell and tissue types (10-13). The functional significance of this modification remains to be elucidated.

1. Bilder, D. and Perrimon, N. (2000) Nature 403, 676-80.
2. Bilder, D. et al. (2000) Science 289, 113-6.
3. Humbert, P.O. et al. (2008) Oncogene 27, 6888-907.
4. Sun, Y. et al. (2009) Mol Biol Cell 20, 3390-400.
5. Qin, Y. et al. (2005) J Cell Biol 171, 1061-71.
6. Navarro, C. et al. (2005) Oncogene 24, 4330-9.
7. Takizawa, S. et al. (2006) Genes Cells 11, 453-64.
8. Nguyen, M.L. et al. (2003) J Virol 77, 6957-64.
9. Yoshihara, K. et al. (2011) Exp Cell Res 317, 413-22.
10. Olsen, J.V. et al. (2010) Sci Signal 3, ra3.
11. Han, G. et al. (2010) Electrophoresis 31, 1080-9.
12. Brill, L.M. et al. (2009) Cell Stem Cell 5, 204-13.
13. Wang, Y.T. et al. (2010) J Proteome Res 9, 5582-97.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.