DNMT3L Antibody (Mouse Specific)

• 产品编号：CST-12309S      品牌：Cell Signaling Technology       原厂货号：12309S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DNMT3L Antibody (Mouse Specific) 能够检测内源性DNMT3L总蛋白水平该多克隆抗体是采用合成的与鼠源DNMT3L蛋白Pro375残基周围序列相对应的肽段免疫动物而生产的。并经过Protein A和多肽亲和层析纯化。哺乳动物细胞中DNA胞嘧啶甲基化是一种可遗传的表观遗传修饰，并且对于基因表达、基因组印记和发育的正确调控都是至关重要的。目前已经确认了3中哺乳动物甲基转移酶家族：DNMT1、DNMT2和 DNMT3 （1,2）。DNMT1在增殖细胞中为组成型表达，是一种维持型甲基转移酶，负责为复制过程中新合成的DNA转移正确的甲基化模式。DNMT3A和DNMT3B 在胚胎干细胞中强烈表达而在成熟的体细胞组织中表达减弱。他们属于从头甲基转移酶，能够甲基化DNA上之前没有被甲基化的区域。DNMT2在成熟体细胞组织中的表达水平较低，它的失活无论是对从头DNA甲基化还是维持型DNA甲基化都没有影响。DNMT3L是DNMT3A 和 DNMT3B从头甲基转移酶的催化失活型调控因子，从而让他们在胚胎干细胞、睾丸、卵巢和胸腺中呈低水平表达（1,2）。这些从头甲基转移酶是一个异源四聚体复合物，包含两分子DNMT3L以及两分子DNMT3A或DNMT3B（3）。DNMT3L包含一个氨基末端ATRX-DNMT3-DNMT3L (ADD)结构域以及羧基端甲基转移酶样结构域（4-7）。甲基转移酶样结构域结合到DNMT3A 和 DNMT3B上通过提高S-腺苷甲硫氨酸和DNA的结合从而促进催化活性（4,5）。ADD结构域能够通过与非甲基化组蛋白H3 Lys4从而将甲基转移酶复合物募集到基因组上转录失活的区域（6,7）。

1. Hermann, A. et al. (2004) Cell Mol Life Sci 61, 2571-87.
2. Turek-Plewa, J. and Jagodziński, P.P. (2005) Cell Mol Biol Lett 10, 631-47.
3. Jia, D. et al. (2007) Nature 449, 248-51.
4. Holz-Schietinger, C. and Reich, N.O. (2010) J Biol Chem 285, 29091-100.
5. Suetake, I. et al. (2004) J Biol Chem 279, 27816-23.
6. Ooi, S.K. et al. (2007) Nature 448, 714-7.
7. Otani, J. et al. (2009) EMBO Rep 10, 1235-41.

原厂资料：

Specificity / Sensitivity

DNMT3L Antibody (Mouse Specific) recognizes endogenous levels of total DNMT3L protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro375 of mouse DNMT3L protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and development (1,2). Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2, and DNMT3 (1,2). DNMT1 is constitutively expressed in proliferating cells and functions as a maintenance methyltransferase, transferring proper methylation patterns to newly synthesized DNA during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of DNA. DNMT2 is expressed at low levels in adult somatic tissues and its inactivation affects neither de novo nor maintenance DNA methylation. 
 
 DNMT3L is a catalytically inactive regulatory factor for the DNMT3A and DNMT3B de novo methyltransferases that is expressed at low levels in embryonic stem cells, testis, ovaries, and thymus (1,2). These de novo methyltransferases consist of a heterotetrameric complex containing two molecules of DNMT3L, and either two molecules of DNMT3A or DNMT3B (3). DNMT3L contains an amino-terminal ATRX-DNMT3-DNMT3L (ADD) domain and a carboxy-terminal methyltransferase-like domain (4-7). The methyltransferase-like domain binds to DNMT3A and DNMT3B to stimulate catalytic activity by increasing the binding of S-adenosylmethionine and DNA (4,5). The ADD domain recruits the methyltransferase complex to transcriptionally inactive regions of the genome by binding to unmethylated histone H3 Lys4 (6,7).

1. Hermann, A. et al. (2004) Cell Mol Life Sci 61, 2571-87.
2. Turek-Plewa, J. and Jagodziński, P.P. (2005) Cell Mol Biol Lett 10, 631-47.
3. Jia, D. et al. (2007) Nature 449, 248-51.
4. Holz-Schietinger, C. and Reich, N.O. (2010) J Biol Chem 285, 29091-100.
5. Suetake, I. et al. (2004) J Biol Chem 279, 27816-23.
6. Ooi, S.K. et al. (2007) Nature 448, 714-7.
7. Otani, J. et al. (2009) EMBO Rep 10, 1235-41.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.