APC2 Antibody

• 产品编号：CST-12301S      品牌：CST       原厂货号：12301S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

APC2抗体识别内源性的APC2总蛋白。该多克隆抗体经合成与人APC2蛋白 Lys458邻近的氨基酸残基序列一致的肽段，免疫动物产生。抗体由蛋白A和肽亲和层析纯化。所有真核细胞的细胞增殖严格依赖于泛素连接酶（E3）APC/C的活性，它的主要功能在于激发细胞周期由分裂中期向分裂后期的转变。APC/C是在分裂间期细胞核中发现的一种1.5 MDa蛋白复合物。这一复合物通过细胞浆扩散，并在有丝分裂中与部分纺锤体有关。APC/C通过促进底物蛋白聚泛素链的组装而发挥多种功能，主要是通过26S蛋白酶体使这些蛋白降解(1,2)。在人类，12种不同的APC/C亚基已经被确定。像所有E3酶一样，APC/C利用已经被E1酶活化的泛素，然后转化为E2酶。确实，APC/C已经被证实短暂地与UBCH5和UBCH10 E2酶相互作用，部分经过包含有环指结构域的亚基APC11 (3-5)。除外E2酶，APC/C的活性严格地依赖于一些辅助因素，与APC/C所处的细胞周期特殊阶段有关。最好的研究是有关Cdc20 和Cdh1/FZR1，包含有C-末端WD40结构域，并通过这些底物(6)中特殊的识别元件D-boxes (7) 和KEN-boxes (8)参与APC/C底物的识别。APC2是cullin家族(9,10)的成员之一，该家族与RING-H2指形蛋白相互作用，RING-H2指形蛋白与Rbx1/Hrt1/Roc1有关，Rbx1/Hrt1/Roc1又称为APC11形成APC/C的催化亚底物。APC2/11亚复合物募集E2酶，诸如UBE2C/UBCH10，并需要APC/C催化底物泛素化(11)。因此，APC是cullin-RING finger-based泛素连接酶的扩展家族成员之一。APC2的生理学重要性在于，APC2的破坏导致小鼠胚胎的死亡(12)。

1. Qiao, X. et al. (2010) Cell Cycle 9, 3904-12.
2. Harper, J.W. et al. (2002) Genes Dev 16, 2179-206.
3. Carroll, C.W. and Morgan, D.O. (2002) Nat Cell Biol 4, 880-7.
4. Gmachl, M. et al. (2000) Proc Natl Acad Sci U S A 97, 8973-8.
5. Leverson, J.D. et al. (2000) Mol Biol Cell 11, 2315-25.
6. Kraft, C. et al. (2005) Mol Cell 18, 543-53.
7. Glotzer, M. et al. (1991) Nature 349, 132-8.
8. Pfleger, C.M. and Kirschner, M.W. (2000) Genes Dev 14, 655-65.
9. Zachariae, W. et al. (1998) Science 279, 1216-9.
10. Yu, H. et al. (1998) Science 279, 1219-22.
11. Tang, Z. et al. (2001) Mol Biol Cell 12, 3839-51.
12. Wirth, K.G. et al. (2004) Genes Dev 18, 88-98.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Hamster, Bovine, Dog, Guinea Pig

Specificity / Sensitivity

APC2 Antibody recognizes endogenous levels of total APC2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys458 of human APC2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Cell proliferation in all eukaryotic cells depends strictly upon the ubiquitin ligase (E3) activity of the anaphase promoting complex/cyclosome (APC/C), whose main function is to trigger the transition of the cell cycle from metaphase to anaphase. APC/C is a 1.5 MDa protein complex found in the nucleus of interphase cells. This complex diffuses throughout the cytoplasm and associates with parts of the spindle apparatus during mitosis. APC/C performs its various functions by promoting the assembly of polyubiquitin chains on substrate proteins, which targets these proteins for degradation by the 26S proteasome (1,2). In humans, twelve different APC/C subunits have been identified. Like all E3 enzymes, APC/C utilizes ubiquitin that has been activated by E1 enzymes and then transferred to E2 enzymes. Indeed APC/C has been shown to transiently interact with UBCH5 and UBCH10 E2 enzymes, in part, via the RING-finger domain-containing subunit, APC11 (3-5). In addition to E2 enzymes, APC/C activity is also strictly dependent upon one of several cofactors that associate with APC/C during specific phases of the cell cycle. The best studied of these are Cdc20 and Cdh1/FZR1, which contain a C-terminal WD40 domain and participate in the recognition of APC/C substrates by interacting with specific recognition elements in these substrates (6), called D-boxes (7) and KEN-boxes (8).Anaphase-promoting complex subunit 2 (APC2) is a distant member of the cullin family (9,10) that interacts with a RING-H2 finger protein related to Rbx1/Hrt1/Roc1, called APC11, to form the catalytic subcomplex of the APC/C. The APC2/11 subcomplex recruits E2 enzymes such as UBE2C/UBCH10 and is required for the APC/C to catalyze substrate ubiquitination (11). Therefore, APC is a member of the expanding family of cullin-RING finger-based ubiquitin ligases. The physiologic importance of APC2 was underscored by the finding that disruption of murine Apc2 causes embryonic lethality (12).

1. Qiao, X. et al. (2010) Cell Cycle 9, 3904-12.
2. Harper, J.W. et al. (2002) Genes Dev 16, 2179-206.
3. Carroll, C.W. and Morgan, D.O. (2002) Nat Cell Biol 4, 880-7.
4. Gmachl, M. et al. (2000) Proc Natl Acad Sci U S A 97, 8973-8.
5. Leverson, J.D. et al. (2000) Mol Biol Cell 11, 2315-25.
6. Kraft, C. et al. (2005) Mol Cell 18, 543-53.
7. Glotzer, M. et al. (1991) Nature 349, 132-8.
8. Pfleger, C.M. and Kirschner, M.W. (2000) Genes Dev 14, 655-65.
9. Zachariae, W. et al. (1998) Science 279, 1216-9.
10. Yu, H. et al. (1998) Science 279, 1219-22.
11. Tang, Z. et al. (2001) Mol Biol Cell 12, 3839-51.
12. Wirth, K.G. et al. (2004) Genes Dev 18, 88-98.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.