Pontin/RUVBL1 Antibody

• 产品编号：CST-12300S      品牌：CST       原厂货号：12300S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Pontin/RUVBL1 Antibody能够检测内源性Pontin/RUVBL1总蛋白水平。该多克隆抗体经由合成的围绕人Pontin/RUVBL1蛋白近羧基端的氨基酸肽段免疫动物而生产的。并经过Protein A和多肽亲和层析纯化。Reptin/RuvBL2 和 Pontin/RuvBL1是密切相关的AAA+ (ATPase Associated with diverse cellular Activities)蛋白超家族中的成员。他们被公认为是驱动Holliday连接中分支迁移的细菌RuvB蛋白的同源物（1）。Reptin 和 Pontin作为染色质重塑和修饰复合物的重要组成成分一起发挥功能，该复合物包括INO80, TIP60, SRCAP, 和 Uri1，他们在基因转录的调控中具有关键作用（1,2）。作为重要的转录共调控因子，Reptin 和 Pontin都与致癌性转化有关，包括那些由c-Myc, β-catenin, 以及 E1A驱动的 (2-7)。许多科学研究报道，在选择的癌症亚型中Pontin的表达水平都升高了，包括肝癌和结肠癌（8-10）。有趣的是，报道发现Pontin能够增强TCF/LEF1-β-catenin转录复合物，然而对于这同一种复合物Reptin却是拮抗作用，由此突出了他们各自的功能相互关联但决不重复（5）。研究还认为Pontin和Reptin还在DNA损伤反应中发挥功能。在DNA损伤前，这两个蛋白都会被ATM磷酸化并且都与Rad-3有关系（11），而据报道在DNA损伤反应中Pontin需要TIP60的活性（12）。

1. Jha, S. and Dutta, A. (2009) Mol Cell 34, 521-33.
2. Gallant, P. (2007) Trends Cell Biol 17, 187-92.
3. Huber, O. et al. (2008) Cancer Res 68, 6873-6.
4. Kim, J.H. et al. (2005) Nature 434, 921-6.
5. Bauer, A. et al. (2000) EMBO J 19, 6121-30.
6. Wood, M.A. et al. (2000) Mol Cell 5, 321-30.
7. Dugan, K.A. et al. (2002) Oncogene 21, 5835-43.
8. Blanc, J.F. et al. (2005) Proteomics 5, 3778-89.
9. Lauscher, J.C. et al. (2007) Hum Pathol 38, 978-85.
10. Huber, O. et al. (2008) Cancer Res 68, 6873-6.
11. Matsuoka, S. et al. (2007) Science 316, 1160-6.
12. Jha, S. et al. (2008) Mol Cell Biol 28, 2690-700.

原厂资料：

Specificity / Sensitivity

Pontin/RUVBL1 Antibody recognizes endogenous levels of total Pontin/RUVBL1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Pontin/RUVBL1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Reptin/RuvBL2 and Pontin/RuvBL1 are closely related members of the AAA+ (ATPase Associated with diverse cellular Activities) superfamily of proteins, and are putatively homologous to bacterial RuvB proteins that drive branch migration of Holliday junctions (1). Reptin and Pontin function together as essential components of chromatin remodeling and modification complexes, such as INO80, TIP60, SRCAP, and Uri1, which play key roles in regulating gene transcription (1,2). In their capacity as essential transcriptional co-regulators, Reptin and Pontin have both been implicated in oncogenic transformations, including those driven by c-Myc, β-catenin, and E1A (2-7).

A number of research studies have reported elevated levels of Pontin in selected cancer subtypes, including hepatocellular carcinoma and colon cancer (8-10). Interestingly, Pontin was reported to potentiate the TCF/LEF1-β-catenin transcriptional complex, whereas Reptin was antagonistic to the same complex, highlighting their related, but non-redundant, function (5). Research studies also suggest a functional role for Pontin and Reptin in the DNA damage response. Both proteins are phosphorylated by ATM and Rad3-related following DNA damage (11), while Pontin is reportedly required for TIP60 activity during the DNA damage response (12).

许多科学研究报道，在选择的癌症亚型中Pontin的表达水平都升高了，包括肝癌和结肠癌（8-10）。有趣的是，报道发现Pontin能够

1. Jha, S. and Dutta, A. (2009) Mol Cell 34, 521-33.
2. Gallant, P. (2007) Trends Cell Biol 17, 187-92.
3. Huber, O. et al. (2008) Cancer Res 68, 6873-6.
4. Kim, J.H. et al. (2005) Nature 434, 921-6.
5. Bauer, A. et al. (2000) EMBO J 19, 6121-30.
6. Wood, M.A. et al. (2000) Mol Cell 5, 321-30.
7. Dugan, K.A. et al. (2002) Oncogene 21, 5835-43.
8. Blanc, J.F. et al. (2005) Proteomics 5, 3778-89.
9. Lauscher, J.C. et al. (2007) Hum Pathol 38, 978-85.
10. Huber, O. et al. (2008) Cancer Res 68, 6873-6.
11. Matsuoka, S. et al. (2007) Science 316, 1160-6.
12. Jha, S. et al. (2008) Mol Cell Biol 28, 2690-700.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.