CUEDC2 Antibody

• 产品编号：CST-12294S      品牌：CST       原厂货号：12294S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

UEDC2抗体可以识别内源性CUEDC2总蛋白。.多抗由合成肽段免疫动物产生，该肽段与人CUEDC2蛋白羧基端肽段序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。CUE domain-containing 2 (CUEDC2)蛋白调控多种细胞事件包括细胞周期调控（1）和炎症反应（2）。研究被表面CUEDC2在多种肿瘤中表达上调，提示钙蛋白可能在肿瘤形成过程中发挥了关键作用（1，3）。110位丝氨酸被Cdk1磷酸化后，CUEDC2会在有丝分裂早期被激活。磷酸化的CUEDC2和Cdc20结合，使anaphase-promoting complex/cyclosome (APC/C)从检验点抑制中被释放，启动细胞周期后期。随后在细胞退出有丝分裂时CUEDC2会被去磷酸化（1）。CUEDC2也是IKKα 和IKKβ活性（2）以及Jak1/Stat3信号通路（4）的抑制因子。研究指出CUEDC2失调后可能导致染色体不稳定从而促进肿瘤发展（1）。许多研究报道指出CUEDC2能下调孕酮受体和雌激素受体α，破坏孕酮受体在乳腺癌细胞中的生长。相反的，也有研究表明CUEDC2和HER2的表达与乳腺癌的发生有重要的关联，促使研究人员认为CUEDC2可能是乳腺癌治疗过程中的重要八点（3,5）。

1. Gao, Y.F. et al. (2011) Nat Cell Biol 13, 924-33.
2. Li, H.Y. et al. (2008) Nat Immunol 9, 533-41.
3. Pan, X. et al. (2011) Nat Med 17, 708-14.
4. Zhang, W.N. et al. (2012) J Biol Chem 287, 382-92.
5. Zhang, P.J. et al. (2007) EMBO J 26, 1831-42.

原厂资料：

Specificity / Sensitivity

CUEDC2 Antibody recognizes endogenous levels of total CUEDC2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CUEDC2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

CUE domain-containing 2 (CUEDC2) protein is involved in regulating many cellular events including cell cycle regulation (1) and inflammation (2). Research studies have shown that CUEDC2 is highly expressed in many types of tumors, suggesting this protein may play a role in tumorigenesis (1,3). CUEDC2 is activated in early mitosis when it is phosphorylated by Cdk1 at Ser110. Phosphorylated CUEDC2 binds to Cdc20, which leads to the release of the anaphase-promoting complex/cyclosome (APC/C) from checkpoint inhibition, initiating anaphase. CUEDC2 is then dephosphorylated when cells exit mitosis (1). CUEDC2 is also an inhibitor of IKKα and IKKβ activation (2) as well as Jak1/Stat3 signaling (4). Research indicates that inappropriate regulation of CUEDC2 may contribute to tumor development by causing chromosome instability (1). Multiple studies have reported that CUEDC2 plays a role in the downregulation of progesterone receptor and estrogen receptor α, impairing the effects of progesterone on breast cancer cell growth. Conversely, research studies have shown that CUEDC2 and HER2 expression have a significant positive correlation in breast cancers, leading investigators to suggest that CUEDC2 could be an important target for breast cancer therapy (3,5).

1. Gao, Y.F. et al. (2011) Nat Cell Biol 13, 924-33.
2. Li, H.Y. et al. (2008) Nat Immunol 9, 533-41.
3. Pan, X. et al. (2011) Nat Med 17, 708-14.
4. Zhang, W.N. et al. (2012) J Biol Chem 287, 382-92.
5. Zhang, P.J. et al. (2007) EMBO J 26, 1831-42.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.