CARD9 Antibody (Mouse Preferred)

• 产品编号：CST-12283S      品牌：CST       原厂货号：12283S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CARD9抗体(优先用于小鼠)能够检测内源性CARD9总蛋白水平。此多克隆抗体通过合成肽免疫动物制备，这种合成肽与鼠源CARD9蛋白His478周围残基相一致。抗体通过蛋白A和多肽亲和层析纯化。CARD9是骨髓细胞中表达的含有细胞凋亡蛋白酶招募域(CARD)的接头蛋白（1,2），在通过C-型凝集素受体(CLRs)如Dectin-1对病原体的识别并介导抗真菌免疫下游信号传递中所必需。通过CLRs对真菌细胞壁糖类的识别可以导致酪氨酸激酶Syk的活化，从而活化PKCδ(5,6)。PKCδ磷酸化CARD9，导致CARD9和Bcl10复合物的形成(6)。这种复合物能够激活NF-κB，导致炎症细胞因子的上调，这对适应性免疫的启动有着重要的作用(3,4,6,7)。CARD9对IL-1β、下游的病毒核酸传感器RIG-I，以及活性氧的产生的诱导起着重要的作用，活性氧的产生对于巨噬细胞内细菌的清除具有重要的作用(2,8)。

1. Bertin, J. et al. (2000) J Biol Chem 275, 41082-6.
2. Hsu, Y.M. et al. (2007) Nat Immunol 8, 198-205.
3. Gross, O. et al. (2006) Nature 442, 651-6.
4. Bi, L. et al. (2010) J Biol Chem 285, 25969-77.
5. Rogers, N.C. et al. (2005) Immunity 22, 507-17.
6. Strasser, D. et al. (2012) Immunity 36, 32-42.
7. LeibundGut-Landmann, S. et al. (2007) Nat Immunol 8, 630-8.
8. Wu, W. et al. (2009) Nat Immunol 10, 1208-14.

原厂资料：

Specificity / Sensitivity

CARD9 Antibody (Mouse Preferred) recognizes endogenous levels of total CARD9 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His478 of mouse CARD9 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

CARD9 is a caspase recruitment domain (CARD)-containing adaptor protein expressed by myeloid cells (1,2). It is required for antifungal immunity downstream of pathogen detection by C-type lectin receptors (CLRs) such as Dectin-1 (3,4). Recognition of carbohydrates on fungal cell walls by CLRs leads to activation of the tyrosine kinase Syk, followed by activation of PKCδ (5,6). PKCδ phosphorylates CARD9, enabling the assembly of a complex containing CARD9 and Bcl10 (6). This complex activates NF-κB, resulting in upregulation of inflammatory cytokines important for initiation of adaptive immunity (3,4,6,7). CARD9 was also shown to be important for the induction of IL-1β, downstream of the viral nucleic acid sensor RIG-I, as well as for the generation of reactive oxygen species important for bacterial killing by macrophages (2,8).

1. Bertin, J. et al. (2000) J Biol Chem 275, 41082-6.
2. Hsu, Y.M. et al. (2007) Nat Immunol 8, 198-205.
3. Gross, O. et al. (2006) Nature 442, 651-6.
4. Bi, L. et al. (2010) J Biol Chem 285, 25969-77.
5. Rogers, N.C. et al. (2005) Immunity 22, 507-17.
6. Strasser, D. et al. (2012) Immunity 36, 32-42.
7. LeibundGut-Landmann, S. et al. (2007) Nat Immunol 8, 630-8.
8. Wu, W. et al. (2009) Nat Immunol 10, 1208-14.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.