Synaptophysin (7H12) Mouse mAb

• 产品编号：CST-12270S      品牌：CST       原厂货号：12270S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Synaptophysin (7H12) Mouse mAb鼠单抗能够检测内源性的突触素总蛋白。该单克隆抗体是由合成的人源突触素蛋白C末端肽段免疫动物而制备的。突触素（Synaptophysin，SYP）是一种神经突触小泡糖蛋白，在神经内分泌细胞和肿瘤中表达（1）。突触素含有4个跨膜结构域，形成一个六聚体通道或缝隙连接样的孔（2）。突触素结合到SNARE蛋白上，突触素/VAMP，防止突触小泡融合复合体中突触素包涵体的形成，积累突触素以供突触活动增加时使用（3）。突触素也负责突触素2/VAMP2靶向定位至突触小泡，其为融合复合物的关键组分（4）。

1. Wiedenmann, B. et al. (1986) Proc Natl Acad Sci USA 83, 3500-4.
2. Valtorta, F. et al. (2004) Bioessays 26, 445-53.
3. Arthur, C.P. and Stowell, M.H. (2007) Structure 15, 707-14.
4. Bonanomi, D. et al. (2007) Biochem J 404, 525-34.

原厂资料：

Specificity / Sensitivity

Synaptophysin (7H12) Mouse mAb recognizes endogenous levels of total synaptophysin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human synaptophysin protein.

Background

Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms (1). Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2). Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3). Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex (4).

1. Wiedenmann, B. et al. (1986) Proc Natl Acad Sci USA 83, 3500-4.
2. Valtorta, F. et al. (2004) Bioessays 26, 445-53.
3. Arthur, C.P. and Stowell, M.H. (2007) Structure 15, 707-14.
4. Bonanomi, D. et al. (2007) Biochem J 404, 525-34.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.