CPT1A (D3B3) Rabbit mAb

• 产品编号：CST-12252S      品牌：CST       原厂货号：12252S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CPT1A (D3B3) Rabbit mAb兔单抗 能够检测内源性CPT1A总蛋白水平。该单克隆抗体是由合成的人源CPT1A蛋白亮氨酸(213位)肽段免疫动物而制备的。肉碱棕榈酰转移酶1（CPT1）位于线粒体外膜，能够转运脂肪酸穿过线粒体膜，以及催化β-氧化的限速步骤（1，2）。这种酶有三种异构体：CPT1A（肝）CPT1B的（肌肉）和CPT1C（脑）（1，2）。缺乏CPT1A会引起一种常染色体隐性遗传的线粒体脂肪酸氧化障碍（3）。研究表明，在人类肌肉中，生理性高血糖和胰岛素水平会抑制CPT1B活性，进而使长链脂肪酸以甘油三酯形式贮存到人体肌肉（4）。此外，缺乏CPT1C的小鼠会有食物摄入量减少和体重减少的表现（5）。这些发现表明，CPT1可能在代谢综合症中发挥作用。

1. Wolfgang, M.J. et al. (2006) Proc Natl Acad Sci U S A 103, 7282-7.
2. Bonnefont, J.P. et al. Mol Aspects Med 25, 495-520.
3. Ogawa, E. et al. (2002) J Hum Genet 47, 342-7.
4. Rasmussen, B.B. et al. (2002) J Clin Invest 110, 1687-93.
5. Wolfgang, M.J. and Lane, M.D. (2011) FEBS J 278, 552-8.

原厂资料：

Specificity / Sensitivity

CPT1A (D3B3) Rabbit mAb recognizes endogenous levels of total CPT1A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu213 of human CPT1A protein.

Background

Carnitine palmitoyltransferase-1 (CPT1), localized to the mitochondrial outer membrane, translocates fatty acids across the mitochondrial membranes and catalyzes the rate-limiting step of β-oxidation (1, 2). There are three isoforms of this enzyme: CPT1A (liver), CPT1B (muscle), and CPT1C (brain) (1, 2). Deficiency of CPT1A results in an autosomal recessive mitochondrial fatty acid oxidation disorder (3). Studies have shown that physiological high blood glucose and insulin levels inhibit CPT1B activity in human muscle and therefore divert long-chain fatty acids toward storage in human muscle as triglycerides (4). Furthermore, mice deficient in CPT1C show less food intake and reduced body weight (5). These findings suggest that CPT1 may play a role in metabolic syndromes.

1. Wolfgang, M.J. et al. (2006) Proc Natl Acad Sci U S A 103, 7282-7.
2. Bonnefont, J.P. et al. Mol Aspects Med 25, 495-520.
3. Ogawa, E. et al. (2002) J Hum Genet 47, 342-7.
4. Rasmussen, B.B. et al. (2002) J Clin Invest 110, 1687-93.
5. Wolfgang, M.J. and Lane, M.D. (2011) FEBS J 278, 552-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.