Phospho-M-CSF Receptor (Tyr699) (D10B11) Rabbit mAb

• 产品编号：CST-12251S      品牌：CST       原厂货号：12251S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

只有当Tyr699磷酸化时，磷酸化M-CSF Receptor (Tyr699) (D10B11) 兔单抗能够检测内源性水平的M-CSF受体。该抗体可能与其他激活的酪氨酸激酶包括PDGF受体和EGFR发生交叉反应。该单克隆抗体是通过用合成磷酸肽免疫动物而制备的，该合成磷酸肽是人M-CSF受体蛋白Tyr699附近的残基。巨噬细胞集落刺激因子（M-CSF, CSF-1）受体是由原癌基因c-fms编码的一种膜嵌合的酪氨酸激酶。M-CSF 受体表达在单核细胞（巨噬细胞及其前体细胞）上并促进这一系列血细胞的生长和发育(1-3)。M-CSF和它的受体结合后会引起受体二聚化、激活、以及细胞质内作为含SH2信号蛋白结合位点的酪氨酸残基自磷酸化(4)。至少有5个主要的酪氨酸自磷酸化位点。Tyr723 (小鼠Tyr721)位于激酶插入（KI）区。磷酸化的Tyr723能够与PI3K激酶的p85亚基以及PLC-γ 2结合(5)。磷酸化的Tyr809能够为Shc提供停泊位点（5）。该受体的过度激活会导致各种细胞体系呈现出恶性特征（6）。激活的M-CSF 受体表明晚期上皮样卵巢癌（7）和乳腺癌预后不良（8）。CSF受体中Tyr669的磷酸化是在Cell Signaling Technology用PhosphoScan®, CST's LC-MS/MS平台确定，该平台用于磷酸化位点的发现（10）。激酶插入（KI）域Tyr699的自磷酸化好像为Grb2衔接蛋白提供了一个结合位点（9）。

1. Stanley, E.R. et al. (1978) Nature 274, 168-70.
2. Byrne, P.V. et al. (1981) J Cell Biol 91, 848-53.
3. Bourette, R.P. and Rohrschneider, L.R. (2000) Growth Factors 17, 155-66.
4. Novak, U. et al. (1996) Oncogene 13, 2607-13.
5. Bourette, R.P. et al. (1997) EMBO J 16, 5880-93.
6. Morley, G.M. et al. (1999) Oncogene 18, 3076-84.
7. Toy, E.P. et al. (2001) Gynecol Oncol 80, 194-200.
8. Maher, M.G. et al. (1998) Clin Cancer Res 4, 1851-6.
9. Hamilton, J.A. (1997) J Leukoc Biol 62, 145-55.
10. Downing, J.R. et al. (1991) Mol Cell Biol 11, 2489-95.

原厂资料：

Specificity / Sensitivity

Phospho-M-CSF Receptor (Tyr699) (D10B11) Rabbit mAb detects endogenous levels of M-CSF receptor only when phosphorylated at Tyr699. This antibody may cross-react with other activated tyrosine kinases including PDGF receptors and EGFR.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr699 of human M-CSF Receptor protein.

Background

Macrophage-colony stimulating factor (M-CSF, CSF-1) receptor is an integral membrane tyrosine kinase encoded by the c-fms proto-oncogene. M-CSF receptor is expressed in monocytes (macrophages and their progenitors) and drives growth and development of this blood cell lineage. (1-3). Binding of M-CSF to its receptor induces receptor dimerization, activation and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins (4). There are at least five major tyrosine autophosphorylation sites. Tyr723 (Tyr721 in mouse) is located in the kinase insert (KI) region. Phosphorylated Tyr723 binds the p85 subunit of PI3 kinase as well as PLCγ2 (5). Phosphorylation of Tyr809 provides a docking site for Shc (5). Overactivation of this receptor can lead to a malignant phenotype in various cell systems (6). The activated M-CSF receptor has been shown to be a predictor of poor outcome in advanced epithelial ovarian carcinoma (7) and breast cancer (8).Phosphorylation of M-CSF receptor on Tyr669 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery, as well as in another publication (10). Autophosphorylation at Tyr699 in the kinase insert (KI) domain appears to provide a binding site for the Grb2 adaptor protein (9).

1. Stanley, E.R. et al. (1978) Nature 274, 168-70.
2. Byrne, P.V. et al. (1981) J Cell Biol 91, 848-53.
3. Bourette, R.P. and Rohrschneider, L.R. (2000) Growth Factors 17, 155-66.
4. Novak, U. et al. (1996) Oncogene 13, 2607-13.
5. Bourette, R.P. et al. (1997) EMBO J 16, 5880-93.
6. Morley, G.M. et al. (1999) Oncogene 18, 3076-84.
7. Toy, E.P. et al. (2001) Gynecol Oncol 80, 194-200.
8. Maher, M.G. et al. (1998) Clin Cancer Res 4, 1851-6.
9. Hamilton, J.A. (1997) J Leukoc Biol 62, 145-55.
10. Downing, J.R. et al. (1991) Mol Cell Biol 11, 2489-95.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.