Skp1 (D3J4N) Rabbit mAb

• 产品编号：CST-12248S      品牌：CST       原厂货号：12248S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Skp1 (D3J4N) Rabbit mAb兔单抗识别内源性的Skp1总蛋白。该单克隆抗体经合成与人Skp1蛋白羧基末端邻近氨基酸残基序列一致的肽段，多肽免疫动物产生。泛素通过泛素化作用可以与许多细胞蛋白共价连接，这些蛋白可以被26S蛋白酶体降解。3种组分包含在蛋白-泛素共价连接过程。泛素首先通过形成具有活化组分E1的硫酯复合物被激活；活化的泛素随后转运到泛素载体蛋白E2，随后从E2到泛素连接酶E3进行最后的转运到epsilon-NH2的靶蛋白赖氨酸残基(1-3)。不同E2和E3蛋白的相互作用导致底物的特异性(4)。最近的数据表明活化的E2短暂地与E3相关，解离是去泛素化的重要步骤(5)。S期激酶相关蛋白(Skp1)是Skp1/CUL1/F-box (SCF) E3泛素连接酶蛋白复合物的一种重要的支架蛋白。多种F-box蛋白(例如：β-TrCP, Skp2)介导与Skp1的相互作用，经它们确定的40氨基酸的保守结构域，与被泛素化的底物相互作用(例如：β-catenin, p27) (4)。

1. Ciechanover, A. (1998) EMBO J. 17, 7151-60.
2. Hochstrasser, M. (2000) Nat. Cell Biol. 2, E153-7.
3. Hochstrasser, M. (2000) Science 289, 563-4.
4. DeSalle, L.M. and Pagano, M. (2001) FEBS Lett. 490, 179-89.
5. Deffenbaugh, A.E. et al. (2003) Cell 114, 611-22.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Zebrafish

Specificity / Sensitivity

Skp1 (D3J4N) Rabbit mAb recognizes endogenous levels of total Skp1 protein.

Background

Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2 and then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). Recent data suggests that activated E2 associates transiently with E3, and the dissociation is a critical step for ubiquitination (5). S phase kinase-associated protein 1 (Skp1) is a critical scaffold protein of the Skp1/CUL1/F-box (SCF) E3 ubiquitin ligase protein complex. Various F-box proteins (e.g., β-TrCP, Skp2) mediate an interaction with Skp1, via their defining and conserved domain of 40 amino acids, and with substrates to be ubiquitinated (e.g., β-catenin, p27) (4).

1. Ciechanover, A. (1998) EMBO J. 17, 7151-60.
2. Hochstrasser, M. (2000) Nat. Cell Biol. 2, E153-7.
3. Hochstrasser, M. (2000) Science 289, 563-4.
4. DeSalle, L.M. and Pagano, M. (2001) FEBS Lett. 490, 179-89.
5. Deffenbaugh, A.E. et al. (2003) Cell 114, 611-22.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody