TPX2 (D2R5C) XP? Rabbit mAb

• 产品编号：CST-12245P      品牌：Cell Signaling Technology       原厂货号：12245P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

.TPX2 (D2R5C) XP® Rabbit mAb可以识别内源性TPX2总蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人TPX2的420位赖氨酸邻近残基序列一致。核膜形成，有丝分裂纺锤体的聚集以及核转运过程都涉及了Ras家族小GTPase Ran蛋白（1.2）。TPX2，活化Ran（RanGTP）的一个靶蛋白是一个微管核形成蛋白。TPX2与细胞核输入-alpha结合时是失活状态。RanGTP的活性能够破坏这种结合，减弱TPX2的抑制作用（3）。TPX2的结合会激活Aurora A激酶促进其定位到有丝分裂的纺锤体（4,5）。有丝分裂时DNA损伤会丢失Aurora A和TPX2的结合从而使Aurora 激酶失活（6）。TPX2在胰腺癌细胞中高表达，敲除TPX2会增强细胞对紫杉醇的敏感性（7）。

1. Quimby, B.B. and Dasso, M. (2003) Curr Opin Cell Biol 15, 338-44.
2. Hetzer, M. et al. (2002) Nat Cell Biol 4, E177-84.
3. Gruss, O.J. and Vernos, I. (2004) J Cell Biol 166, 949-55.
4. Kufer, T.A. et al. (2002) J Cell Biol 158, 617-23.
5. Bayliss, R. et al. (2004) Cell Cycle 3, 404-7.
6. Bhatia, P. et al. (2010) Cell Cycle 9, 4592-9.
7. Warner, S.L. et al. (2009) Clin Cancer Res 15, 6519-28.

原厂资料：

Specificity / Sensitivity

TPX2 (D2R5C) XP® Rabbit mAb recognizes endogenous levels of total TPX2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys420 of human TPX2 protein.

Background

The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle, and nuclear transport (1,2). TPX2, a target of active Ran (RanGTP), is a microtubule nucleating protein. TPX2 is inactive when bound to nuclear importin-alpha. RanGTP activity disrupts this interaction, relieving inhibition of TPX2 (3). TPX2 binding activates Aurora A kinase and promotes its localization to the mitotic spindle (4,5). DNA damage in mitosis leads to loss of interaction between Aurora A and TPX2 and inactivation of Aurora A kinase (6). TPX2 is highly expressed in pancreatic cancer cells, and knockdown of TPX2 expression in these cells is associated with increased sensitivity to paclitaxel (7).

1. Quimby, B.B. and Dasso, M. (2003) Curr Opin Cell Biol 15, 338-44.
2. Hetzer, M. et al. (2002) Nat Cell Biol 4, E177-84.
3. Gruss, O.J. and Vernos, I. (2004) J Cell Biol 166, 949-55.
4. Kufer, T.A. et al. (2002) J Cell Biol 158, 617-23.
5. Bayliss, R. et al. (2004) Cell Cycle 3, 404-7.
6. Bhatia, P. et al. (2010) Cell Cycle 9, 4592-9.
7. Warner, S.L. et al. (2009) Clin Cancer Res 15, 6519-28.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.