Cyclin B1 (D5C10) XP? Rabbit mAb

• 产品编号：CST-12231P      品牌：Cell Signaling Technology       原厂货号：12231P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

细胞周期蛋白 B1 (D5C10) XP®Rabbit mAb可以识别内源性总的细胞周期蛋白B1。该抗体会在某些细胞系中检测到100kDa未知源性的条带。单克隆抗体由合成肽段免疫动物产生，该肽段与人细胞周期蛋白 B1的氨基端邻近残基序列一致。细胞周期蛋白是一类激活特定细胞周期依赖性激酶以推进细胞周期的蛋白质。所有真核细胞要进入细胞周期都是G2/M转换时激活cdc2/cdk1以调控的。该激活作用分成多个步骤开始于调控亚基cdc2/cdk1与cdc2/cdk1结合以形成促有丝分裂因子（MPF）。MPF保持在失活状态直到被cdk活化激酶（CAK）通过cdc2/cdk1磷酸化161位苏氨酸（1,2），cdc25C可以对cdc2/cdk1的14/15位苏氨酸去磷酸化（3-5）。四个细胞周期蛋白B1磷酸化位点（126,128,133和147位丝氨酸）位于胞质滞留信号（CRS）区域，一般认为这四个磷酸化位点可以调控细胞周期蛋白B1在G2/M检验点时期的移位，促进其聚集在细胞核中并起始有丝分裂（6-9）。

1. Lorca, T. et al. (1992) EMBO J 11, 2381-90.
2. Harper, J.W. and Elledge, S.J. (1998) Genes Dev 12, 285-9.
3. Norbury, C. et al. (1991) EMBO J 10, 3321-9.
4. McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.
5. Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001.
6. Toyoshima-Morimoto, F. et al. (2001) Nature 410, 215-20.
7. Li, J. et al. (1997) Proc Natl Acad Sci U S A 94, 502-7.
8. Takizawa, C.G. and Morgan, D.O. (2000) Curr Opin Cell Biol 12, 658-65.
9. Santos, S.D. et al. (2012) Cell 149, 1500-13.
10. Jackman, M. et al. (2003) Nat Cell Biol 5, 143-8.
11. Gong, D. and Ferrell, J.E. (2010) Mol Biol Cell 21, 3149-61.
12. Mashal, R.D. et al. (1996) Cancer Res 56, 4159-63.
13. Kawamoto, H. et al. (1997) Am J Pathol 150, 15-23.
14. Soria, J.C. et al. (2000) Cancer Res 60, 4000-4.

原厂资料：

Specificity / Sensitivity

Cyclin B1 (D5C10) XP® Rabbit mAb recognizes endogenous levels of total cyclin B1 protein. This antibody also detects a 100 kDa protein of unknown origin in some cell lines.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human cyclin B1 protein.

Background

Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) (1,2) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C (3-5). Four cyclin B1 phosphorylation sites (Ser126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis (6-9). While MPF itself can phosphorylate Ser126 and Ser128, polo-like kinase 1 (PLK1) phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 (6,10). At the end of mitosis, cyclin B1 is targeted for degradation by the anaphase-promoting complex (APC), allowing for cell cycle progression (11). Research studies have shown that cyclin B1 is overexpressed in breast, prostate, and non-small cell lung cancers (12-14).

1. Lorca, T. et al. (1992) EMBO J 11, 2381-90.
2. Harper, J.W. and Elledge, S.J. (1998) Genes Dev 12, 285-9.
3. Norbury, C. et al. (1991) EMBO J 10, 3321-9.
4. McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.
5. Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001.
6. Toyoshima-Morimoto, F. et al. (2001) Nature 410, 215-20.
7. Li, J. et al. (1997) Proc Natl Acad Sci U S A 94, 502-7.
8. Takizawa, C.G. and Morgan, D.O. (2000) Curr Opin Cell Biol 12, 658-65.
9. Santos, S.D. et al. (2012) Cell 149, 1500-13.
10. Jackman, M. et al. (2003) Nat Cell Biol 5, 143-8.
11. Gong, D. and Ferrell, J.E. (2010) Mol Biol Cell 21, 3149-61.
12. Mashal, R.D. et al. (1996) Cancer Res 56, 4159-63.
13. Kawamoto, H. et al. (1997) Am J Pathol 150, 15-23.
14. Soria, J.C. et al. (2000) Cancer Res 60, 4000-4.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.