IL-4 (D19A10) Rabbit mAb

• 产品编号：CST-12227S      品牌：CST       原厂货号：12227S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IL-4 (D19A10) Rabbit mAb兔单抗能够检测内源性IL-4总蛋白水平。此单克隆抗体通过重组人源IL-4蛋白免疫动物制备。IL-4是一种由活化的T细胞、嗜碱性粒细胞以及肥大细胞分泌的细胞因子(1,2)。尽管它有助于多种不同的免疫调节反应，但其主要作为一种负责诱导幼稚T细胞分化为Th2细胞的细胞因子，这种Th2细胞因其能够产生IL-4、IL-5和 IL-10而被定义(3)。另外，IL-4通过诱导B细胞中的IgE的产生，有助于免疫球蛋白的类别转换(4,5)。IL-4通过结合IL-4受体，导致酪氨酸磷酸化并激活Stat6转录因子。

1. Yokota, T. et al. (1986) Proc Natl Acad Sci USA 83, 5894-8.
2. Grabstein, K. et al. (1986) J Exp Med 163, 1405-14.
3. Kopf, M. et al. (1993) Nature 362, 245-8.
4. Kotowicz, K. and Callard, R.E. (1993) Eur J Immunol 23, 2250-6.
5. Thyphronitis, G. et al. (1989) Proc Natl Acad Sci USA 86, 5580-4.
6. Hou, J. et al. (1994) Science 265, 1701-6.

原厂资料：

Specificity / Sensitivity

IL-4 (D19A10) Rabbit mAb recognizes endogenous levels of total IL-4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human IL-4 protein.

Background

Interleukin-4 (IL-4) is a cytokine secreted by activated T cells, basophils, and mast cells (1,2). While it contributes to many immunomodulatory responses, it is mainly recognized as the cytokine responsible for eliciting differentiation of naive T cells into Th2 lineage cells that are defined by their secretion of IL-4, IL-5, and IL-10 (3). In addition, IL-4 contributes to immunoglobulin class switching by inducing the production of IgE from B cells (4,5). IL-4 acts through the IL-4 receptor, leading to tyrosine phosphorylation and activation of the Stat6 transcription factor (6).

1. Yokota, T. et al. (1986) Proc Natl Acad Sci USA 83, 5894-8.
2. Grabstein, K. et al. (1986) J Exp Med 163, 1405-14.
3. Kopf, M. et al. (1993) Nature 362, 245-8.
4. Kotowicz, K. and Callard, R.E. (1993) Eur J Immunol 23, 2250-6.
5. Thyphronitis, G. et al. (1989) Proc Natl Acad Sci USA 86, 5580-4.
6. Hou, J. et al. (1994) Science 265, 1701-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.