HMOX2/HO-2 Antibody

• 产品编号：CST-12226S      品牌：Cell Signaling Technology       原厂货号：12226S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

HMOX2/HO-2 Antibody能够检测内源性HMOX2/HO-2总蛋白水平。该多克隆抗体是由合成的人源HMOX2/HO-2蛋白甲硫氨酸(262位)肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。血红素加氧酶（HMOX或HO）催化血红素氧化降解成铁，一氧化碳和胆绿素的限速步骤（1）。胆绿素随后转换为胆红素（2）。血红素是一种强促氧化剂，而胆红素是一种强抗氧化剂（2）。各种研究表明，血红素加氧酶的失调可能会导致氧化应激相关的疾病（2）。血红素加氧酶有三种同工酶：HMOX1/HO-1，HMOX2/HO-2，HMOX3/HO-3（1,2）。 HMOX1/HO-1被血红素和其他胁迫刺激诱导表达（1,3）。 HMOX2/HO-2和HMOX3/HO-3组成型表达（1,3）。

1. Piotrkowski, B. et al. (2009) J Endocrinol 203, 155-65.
2. Synowiec, E. et al. (2012) Mol Biol Rep 39, 2081-7.
3. Otterbein, L.E. and Choi, A.M. (2000) Am J Physiol Lung Cell Mol Physiol 279, L1029-37.

原厂资料：

Specificity / Sensitivity

HMOX2/HO-2 Antibody recognizes endogenous levels of total HMOX2/HO-2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Met262 of human HMOX2/HO-2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Heme oxygenases (HMOX or HO) catalyze the rate-limiting step of the oxidative degradation of heme into iron, carbon monoxide, and biliverdin (1). Biliverdin is then converted to bilirubin (2). Heme is a strong pro-oxidant whereas bilirubin is a strong antioxidant (2). Research studies suggest disregulation of heme oxygenases may contribute to oxidative stress-related diseases (2). There are three isozymes of heme oxygenases: HMOX1/HO-1, HMOX2/HO-2, and HMOX3/HO-3 (1,2). HMOX1/HO-1 is inducible by heme and other stress stimuli (1,3). HMOX2/HO-2 and HMOX3/HO-3 are constitutively expressed (1,3).

1. Piotrkowski, B. et al. (2009) J Endocrinol 203, 155-65.
2. Synowiec, E. et al. (2012) Mol Biol Rep 39, 2081-7.
3. Otterbein, L.E. and Choi, A.M. (2000) Am J Physiol Lung Cell Mol Physiol 279, L1029-37.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.