MAPK Family Alexa Fluor? 647 Conjugated Antibody Sampler Kit

• 产品编号：CST-12207S      品牌：Cell Signaling Technology       原厂货号：12207S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

该抗体能识别内源性水平的在Thr180和Tyr182位点双重磷酸化的p38促分裂原活化蛋白激酶。此抗体与p44/42 MAPK (Erk1/2)或SAPK/JNK的磷酸化形式没有明显的交叉反应。Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate)能识别内源性水平的p44和p22 MAPK (Erk1/2)，无论在Erk1的Thr202和Tyr204位点双磷酸化（Erk2的Thr185 和Tyr187位点），还是在Thr202位点磷酸化。此抗体与SAPK/JNK或p38 MAPK磷酸化的残基没有交叉反应。p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Alexa Fluor® 647 Conjugate)能识别内源性水平的总p44/42 MAPK (Erk1/2)蛋白。此抗体与SAPK/JNK或p38 MAPK没有交叉反应。Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Alexa Fluor® 647 Conjugate)能识别内源性水平的在Thr183和Tyr185位点双磷酸化的p46和p54 SAPK/JNK。此抗体不能识别内源性水平的磷酸化的p44/42或p38 MAPK。单克隆抗体通过用合成的磷酸肽或合成肽免疫动物制备，该合成的磷酸肽是人p38 MAPK蛋白Thr180/Tyr182周围的残基、人p44 MAPK蛋白Thr202/Tyr204周围的残基、人SAPK/JNK蛋白Thr183/Tyr185周围的残基，该合成肽是大鼠p44 MAPK蛋白羧基端附近的残基。该试剂盒提供了一种经济的方法来研究促分裂原活化蛋白激酶蛋白家族成员的活化状态，不需要荧光二抗。促分裂原活化蛋白激酶（MAPKs）是一种广泛保守的丝氨酸/苏氨酸蛋白激酶家族，与很多细胞程序有关，如细胞增殖、分化、运动和死亡。p38促分裂原活化蛋白激酶，也被称为RK（1）或CSBP（2），是酵母HOG激酶（参与信号级联放大控制细胞对细胞因子和应激的反应）的哺乳动物同源基因（1-4）。p44/42 MAPK (Erk1/2)信号通路可以被许多细胞外刺激激活，包括有丝分裂原、生长因子和细胞因子（5-7）。研究者认为它是癌症诊断和治疗中的一个重要靶点（8）。应激活化蛋白激酶/ Jun氨基末端激酶(SAPK/JNK)能被多种环境应激有效地激活，包括紫外线和γ射线、神经酰胺类、炎性细胞因子以及某些情况下的生长因子和G蛋白偶联性受体（GPCR）激动剂（9）。

原厂资料：

Specificity / Sensitivity

Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of p38 MAP kinase only when dually phosphorylated at Thr180 and Tyr182. This antibody does not appreciably cross-react with the corresponding phosphorylated forms of either p44/42 MAPK (Erk1/2) or SAPK/JNK. Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of p44 and p42 MAPK (Erk1/2) when either dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), or singly phosphorylated at Thr202. This antibody does not cross-react with the corresponding phosphorylated residues of either SAPK/JNK or p38 MAP kinases. p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total p44/42 MAPK (Erk1/2) protein. This antibody does not cross-react with SAPK/JNK or p38 MAP kinases. Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of p46 and p54 SAPK/JNK dually phosphorylated at Thr183 and Tyr185. This antibody does not recognize endogenous levels of phosphorylated p44/42 or p38 MAP kinases.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr180/Tyr182 of human p38 MAP kinase protein, a synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase protein, a synthetic peptide corresponding to residues near the carboxy terminus of rat p44 MAP kinase protein, or a synthetic phosphopeptide corresponding to residues around Thr183/Tyr185 of human SAPK/JNK protein.

Description

The MAPK Family Alexa Fluor® 647 Conjugated Antibody Sampler Kit provides an economical means to study the activation status of members of the MAPK family of proteins without the need for a fluorescent secondary antibody.

Background

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (5-7), and research investigators consider it an important target in the diagnosis and treatment of cancer (8). The stress-activated protein kinase/Jun-amino-terminal kinase (SAPK/JNK) is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists (9).

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibod-
ies. Protect from light. Do not freeze.