SPT16 (D7I2K) Rabbit mAb

• 产品编号：CST-12191S      品牌：CST       原厂货号：12191S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SPT16 (D7I2K) Rabbit mAb 兔单抗能够检测内源性SPT16总蛋白水平。该单克隆抗体是采用合成的与人源SPT16蛋白Leu662残基周围序列相对应的肽段免疫动物而生产的。Supressor of Ty-16 (SPT16)以及structure-specific recognition protein-1 (SSRP1)是转录延伸中至关重要的染色质转录促进复合物的亚单位（1,2）。FACT能够通过对活跃基因开放阅读框内核小体的去稳定作用来促进RNA聚合酶依赖的转录（3-5）。FACT能够破坏导致H2A-H2B二聚体丢失的组蛋白-组蛋白以及组蛋白-DNA的联系，从而使成为RNA聚合酶转录激活障碍的核小体去稳定（2,3,6）。FACT可能还可作为组蛋白伴侣在RNA聚合酶通路之后重新组装核小体（7）。除了转录，FACT还可以通过CK2促进p53活化以及促进DNA损伤的组蛋白H2AX-H2B交换，从而在酵母的DNA复制和DNA修复中发挥作用（8-10）。

1. Winkler, D.D. and Luger, K. (2011) J Biol Chem 286, 18369-74.
2. Orphanides, G. et al. (1999) Nature 400, 284-8.
3. Orphanides, G. et al. (1998) Cell 92, 105-16.
4. Birch, J.L. et al. (2009) EMBO J 28, 854-65.
5. Orphanides, G. and Reinberg, D. (2000) Nature 407, 471-5.
6. Keller, D.M. and Lu, H. (2002) J Biol Chem 277, 50206-13.
7. Belotserkovskaya, R. et al. (2003) Science 301, 1090-3.
8. Schlesinger, M.B. and Formosa, T. (2000) Genetics 155, 1593-606.
9. Heo, K. et al. (2008) Mol Cell 30, 86-97.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Hamster, Xenopus, Zebrafish, Bovine, Dog, Guinea Pig, Horse

Specificity / Sensitivity

SPT16 (D7I2K) Rabbit mAb recognizes endogenous levels of total SPT16 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu662 of human SPT16 protein.

Background

Supressor of Ty-16 (SPT16) and structure-specific recognition protein-1 (SSRP1) are subunits of the facilitates chromatin transcription (FACT) complex that is essential for transcription elongation (1,2). FACT facilitates RNA polymerase-dependent transcription of chromatin templates by destabilizing the nucleosomes within the open reading frames of active genes (3-5). FACT destabilizes the nucleosomes, which would otherwise act as barriers to RNA polymerase transcription activity, by disrupting histone-histone and histone-DNA contacts that lead to the eviction of the histone H2A-H2B dimer (2,3,6). FACT may also function as a histone chaperone to reassemble nucleosomes after RNA polymerase passage (7). In addition to transcription, FACT activity has been shown to have a role in DNA replication in yeast and in DNA repair by contributing to the activation of p53 by CK2 and by facilitating histone H2AX-H2B exchange upon DNA damage (8-10).

1. Winkler, D.D. and Luger, K. (2011) J Biol Chem 286, 18369-74.
2. Orphanides, G. et al. (1999) Nature 400, 284-8.
3. Orphanides, G. et al. (1998) Cell 92, 105-16.
4. Birch, J.L. et al. (2009) EMBO J 28, 854-65.
5. Orphanides, G. and Reinberg, D. (2000) Nature 407, 471-5.
6. Keller, D.M. and Lu, H. (2002) J Biol Chem 277, 50206-13.
7. Belotserkovskaya, R. et al. (2003) Science 301, 1090-3.
8. Schlesinger, M.B. and Formosa, T. (2000) Genetics 155, 1593-606.
9. Heo, K. et al. (2008) Mol Cell 30, 86-97.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.