HSP60 (D6F1) XP? Rabbit mAb

• 产品编号：CST-12165S      品牌：CST       原厂货号：12165S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

HSP60 (D6F1) XP® Rabbit mAb兔单抗能够检测内源性的 HSP60 总蛋白。该单克隆抗体是经由合成的围绕人 HSP60蛋白 Trp68 的氨基酸肽段免疫动物而生产的。在原核和真核生物生物合成和细胞应激时分子伴侣可以防止蛋白质的错误折叠和堆积（1-3）。HSP60主要被认为是线粒体蛋白，对关键蛋白质转入线粒体后的正确折叠很重要（4）。目前比较清楚的是HSP60在很多细胞的细胞质中存在，并且可以被应激、炎症和免疫反应诱导，自身抗体与老年痴呆、冠心病、多发性硬化和糖尿病均相关（5-8）。

1. Hartl, F.U. (1996) Nature 381, 571-579.
2. Bukau, B. and Horwich, A.L. (1998) Cell 92, 351-366.
3. Hartl, F.U. and Hayer-Hartl, M. (2002) Science 295, 1852-1858.
4. Jindal, S. et al. (1989) Mol. Cell Biol. 9, 2279-2283.
5. Itoh, H. et al. (2002) Eur. J. Biochem. 269, 5931-5938.
6. Gupta, S. and Knowlton, A.A. J. Cell Mol. Med. 9, 51-58.
7. Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11, 116-128.
8. Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292, E292-E297.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Chicken, Dog, Horse

Specificity / Sensitivity

HSP60 (D6F1) XP® Rabbit mAb recognizes endogenous levels of total HSP60 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Trp68 of human HSP60 protein.

Background

In both prokaryotic and eukaryotic cells the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones (1-3). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (4). Research studies have shown that a significant amount of HSP60 is also present in the cytosol of many cells, and that it is induced by stress, inflammatory and immune responses, and autoantibodies correlated with Alzheimer's, coronary artery diseases, MS, and diabetes (5-8).

1. Hartl, F.U. (1996) Nature 381, 571-579.
2. Bukau, B. and Horwich, A.L. (1998) Cell 92, 351-366.
3. Hartl, F.U. and Hayer-Hartl, M. (2002) Science 295, 1852-1858.
4. Jindal, S. et al. (1989) Mol. Cell Biol. 9, 2279-2283.
5. Itoh, H. et al. (2002) Eur. J. Biochem. 269, 5931-5938.
6. Gupta, S. and Knowlton, A.A. J. Cell Mol. Med. 9, 51-58.
7. Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11, 116-128.
8. Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292, E292-E297.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.