p190-A RhoGAP (D8Q6C) Rabbit mAb

• 产品编号：CST-12164S      品牌：CST       原厂货号：12164S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

p190-A RhoGAP (D8Q6C) Rabbit mAb可以识别内源性总的p190-A RhoGAP蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人p190-A RhoGAP蛋白的Asn585及其邻近氨基酸残基序列一致。Rho家族GTPase是包括细胞骨架构建，细胞生长和分化，转录调控，和细胞黏附/运动等多个过程的重要调控因子。这些蛋白的活性最初时通过鸟苷酸交换因子（GEFs）控制的，帮助GDP被GTP交换，促进活性（GTP结合）状态，GTPase激活蛋白（GAPs）促进GTP纾解保持在失活（GDP-结合）状态（1,2）。 p190 RhoGAP蛋白是广泛表达的Rho家族GAPs。P190-A被认为是一种肿瘤抑制因子，研究表明染色体包含p190-A的区域缺失或者重排与癌症的发育相关（3,4）。P190-A与有丝分裂原诱导转录因子TFII-I结合，将它推入细胞质并抑制它的活性。P190-A的Tyr308磷酸化降低了它对TFII-I的亲和力，减弱抑制作用（5）。P190-A也可以小鼠的抑制生长因子-诱导的肿瘤（6）以及影响培养细胞的卵裂沟和胞质分裂（7）。 小鼠缺乏p190-B RhoGAP会表现出过度的Rho活性和转录因子CREB活性降低（8）。细胞的p190-B的缺失也会表现出脂肪形成的缺陷（9）。越来越多的证据表明p190经过酪氨酸磷酸化后会激活它的GAP结构域（9-11）。酪氨酸磷酸化的水平是通过Src过表达增强的（10,11）。IGF-I处理会磷酸化激活p190-B RhoGAP以下调Rho，随之增强脂肪形成过程中的IGF信号通路（9）。

1. Peck, J. et al. (2002) FEBS Lett. 528, 27-34.
2. Moon, S.Y. and Zheng, Y. (2003) Trends Cell Biol. 13, 13-22.
3. Wang, Z. et al. (1996) Cell Growth Differ 7, 123-33.
4. Tikoo, A. et al. (2000) Gene 257, 23-31.
5. Jiang, W. et al. (2005) Mol Cell 17, 23-35.
6. Wolf, R.M. et al. (2003) Genes Dev 17, 476-87.
7. Su, L. et al. (2003) J Cell Biol 163, 571-82.
8. Sordella, R. et al. (2002) Dev Cell 2, 553-65.
9. Sordella, R. et al. (2003) Cell 113, 147-58.
10. Chang, J.H. et al. (1995) J Cell Biol 130, 355-68.
11. Roof, R.W. et al. (1998) Mol Cell Biol 18, 7052-63.

原厂资料：

Specificity / Sensitivity

p190-A RhoGAP (D8Q6C) Rabbit mAb recognizes endogenous levels of total p190-A RhoGAP protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn585 of human p190-A RhoGAP protein.

Background

Rho family GTPases are key regulators of diverse processes such as cytoskeletal organization, cell growth and differentiation, transcriptional regulation, and cell adhesion/motility. The activities of these proteins are controlled primarily through guanine nucleotide exchange factors (GEFs) that facilitate the exchange of GDP for GTP, promoting the active (GTP-bound) state, and GTPase activating proteins (GAPs) that promote GTP hydrolysis and the inactive (GDP-bound) state (1,2). 
 
 The p190 RhoGAP proteins are widely expressed Rho family GAPs. p190-A has been characterized as a tumor suppressor, and research studies have shown that loss or rearrangement of the chromosomal region containing the gene for p190-A is linked to tumor development (3,4). p190-A binds the mitogen-inducible transcription factor TFII-I, sequestering it in the cytoplasm and inhibiting its activity. Phosphorylation of p190-A at Tyr308 reduces its affinity for TFII-I, relieving the inhibition (5). p190-A can also inhibit growth factor-induced gliomas in mice (6) and affect cleavage furrow formation and cytokinesis in cultured cells (7). 
 
 Mice lacking p190-B RhoGAP show excessive Rho activation and a reduction in activation of the transcription factor CREB (8). Cells deficient in p190-B display defective adipogenesis (9). There is increasing evidence that p190 undergoes tyrosine phosphorylation, which activates its GAP domain (9-11). Levels of tyrosine phosphorylation are enhanced by Src overexpression (10,11). IGF-I treatment downregulates Rho through phosphorylation and activation of p190-B RhoGAP, thereby enhancing IGF signaling implicated in adipogenesis (9).

1. Peck, J. et al. (2002) FEBS Lett. 528, 27-34.
2. Moon, S.Y. and Zheng, Y. (2003) Trends Cell Biol. 13, 13-22.
3. Wang, Z. et al. (1996) Cell Growth Differ 7, 123-33.
4. Tikoo, A. et al. (2000) Gene 257, 23-31.
5. Jiang, W. et al. (2005) Mol Cell 17, 23-35.
6. Wolf, R.M. et al. (2003) Genes Dev 17, 476-87.
7. Su, L. et al. (2003) J Cell Biol 163, 571-82.
8. Sordella, R. et al. (2002) Dev Cell 2, 553-65.
9. Sordella, R. et al. (2003) Cell 113, 147-58.
10. Chang, J.H. et al. (1995) J Cell Biol 130, 355-68.
11. Roof, R.W. et al. (1998) Mol Cell Biol 18, 7052-63.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.
Recommended Antibody Dilutions:
Western blotting  1:1000
Immunoprecipitation  1:50