IL-10 (D13A11) XP? Rabbit mAb

• 产品编号：CST-12163S      品牌：CST       原厂货号：12163S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IL-10 (D13A11) Rabbit mAb能够检测内源性IL-10总蛋白水平。此单克隆抗体是通过人源重组IL-10蛋白免疫动物制备的。IL-10 是抗炎细胞因子，它由T细胞、NK细胞和巨噬细胞产生(1,2)。IL-10通过结合由IL-10 RI和 IL-10 RII组成的细胞表面受体复合物而启动信号转导(1)。IL-10的结合导致Jak1和Tyk2的激活, 继而磷酸化Stat-3 (1,3)。IL-10的抗炎活性是由于它能通过其他的细胞因子受体而阻止细胞传递，特别是通过上调SOCS-1的表达而抑制IFNγ受体(1,3)。除此之外, IL-10通过抑制酪氨酸磷酸化CD28而提高T细胞耐受性(4,5)。IL-10是免疫应答的重要负调控因子,这有助于维持免疫细胞的成熟态(1)。相反, IL-10水平的上升对利什曼虫的持续感染有很大的帮助(6)。

1. Pestka, S. et al. (2004) Immunol Rev 202, 8-32.
2. Akuffo, H. et al. (1999) Clin Exp Immunol 117, 529-34.
3. O'Shea, J.J. and Murray, P.J. (2008) Immunity 28, 477-87.
4. Akdis, C.A. and Blaser, K. (2001) Immunology 103, 131-6.
5. Akdis, C.A. et al. (2000) FASEB J 14, 1666-8.
6. Von Stebut, E. Eur J Dermatol 17, 115-22.

原厂资料：

Specificity / Sensitivity

IL-10 (D13A11) Rabbit mAb recognizes endogenous levels of total IL-10 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human IL-10 protein.

Background

Interleukin-10 (IL-10) is an anti-inflammatory cytokine that is produced by T cells, NK cells, and macrophages (1,2). IL-10 initiates signal transduction by binding to a cell surface receptor complex consisting of IL-10 RI and IL-10 RII (1), leading to the activation of Jak1 and Tyk2 and phosphorylation of Stat3 (1,3). The anti-inflammatory activity of IL-10 is due to its ability to block signaling through other cytokine receptors, notably IFN-γ receptor, by upregulating expression of SOCS1 (1,3). In addition, IL-10 promotes T cell tolerance by inhibiting tyrosine phosphorylation of CD28 (4,5). IL-10 is an important negative regulator of the immune response, which allows for maintenance of pregnancy (1). In contrast, increased IL-10 levels contribute to persistent Leishmania major infections (6).

1. Pestka, S. et al. (2004) Immunol Rev 202, 8-32.
2. Akuffo, H. et al. (1999) Clin Exp Immunol 117, 529-34.
3. O'Shea, J.J. and Murray, P.J. (2008) Immunity 28, 477-87.
4. Akdis, C.A. and Blaser, K. (2001) Immunology 103, 131-6.
5. Akdis, C.A. et al. (2000) FASEB J 14, 1666-8.
6. Von Stebut, E. Eur J Dermatol 17, 115-22.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.
Recommended Antibody Dilutions:
Western blotting  1:1000
Flow Cytometry  1:400