Apoptosis and Proliferation Alexa Fluor? 488 Conjugated Antibody Sampler Kit

• 产品编号：CST-12145S      品牌：Cell Signaling Technology       原厂货号：12145S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor® 488 Conjugate)能识别内源性水平的在Asp175位点裂解的caspase-3蛋白。Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate)能识别内源性水平的人PARP1蛋白的大片段（89kDa，由半胱天冬酶裂解产生）。此抗体不识别全长的PARP1或其他PARP亚型。Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate)能识别内源性水平的总Ki-67蛋白。单克隆抗体通过用合成肽或重组蛋白免疫动物制备，该合成肽是人caspase-3蛋白Asp175周围的残基、人PARP蛋白Asp214周围的残基；该重组蛋白特异性针对Ki-67蛋白的氨基端。Apoptosis and Proliferation Alexa Fluor® 488 Conjugated Antibody Sampler Kit通过使用已知的标记，提供了一种经济的方法来研究细胞凋亡和增殖的状态，不需要荧光二抗。Caspase-3 (CPP-32, Apoptain, Yama, SCA-1)是一个关键的细胞凋亡“刽子手”，它部分或完全地为许多重要蛋白（如核酶多腺苷酸（ADP核糖）聚合酶（PARP））的溶蛋白性裂解负责（1）。PARP参与应对环境应激的DNA修复（2）。Ki-67是一种核非组蛋白，通常在增殖的细胞间表达，在静止的细胞中则没有（3,4）。Ki-67能检测G1，S，G2和有丝分裂期的增殖细胞，而不能检测G0静止期的细胞。

原厂资料：

Specificity / Sensitivity

Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of caspase-3 protein only when cleaved at Asp175. Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of the large fragment (89 kDa) of human PARP1 protein produced by caspase cleavage. This antibody does not recognize full length PARP1 or other PARP isoforms. Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total Ki-67 protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp175 of human caspase-3 protein, a synthetic peptide corresponding to residues surrounding Asp214 of human PARP protein, or a recombinant protein specific to the amino terminus of Ki-67 protein.

Description

The Apoptosis and Proliferation Alexa Fluor® 488 Conjugated Antibody Sampler Kit provides an economical means to study apoptosis and proliferation status using known markers without the need for a fluorescent secondary antibody.

Background

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). PARP is involved in DNA repair in response to environmental stress (2). Ki-67 is a nuclear nonhistone protein that is universally expressed among proliferating cells and absent in quiescent cells (3,4). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C.
Do not aliquot the antibodies. Protect from light. Do not freeze.