CDK5 (1H3) Mouse mAb

• 产品编号：CST-12134S      品牌：CST       原厂货号：12134S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CDK5 (1H3) Mouse mAb 鼠单抗能够检测内源性的CDK5总蛋白。该单克隆抗体是由合成的大鼠CDK5蛋白N末端肽段免疫动物而制备的。细胞周期蛋白依赖性激酶（CDKs）是丝氨酸/苏氨酸激酶，它们被周期蛋白激活，控制真核细胞的细胞周期。CDK5与其家庭成员共享着高度同源的序列，它被认为主要是作用于有丝分裂后的神经元以调节这些细胞的细胞结构。与周期蛋白类似，尽管它们缺乏序列同源性，调节亚基p35和p39与CDK5结合并激活CDK5。CDK5是广泛表达的，由于在有丝分裂后神经元中P35和P39具有窄表达谱，CDK5主要在中枢神经系统中具有高水平的激酶活性。大量CDK5的底物已被确定，但尚未发现某个底物是P35或P39特异的。CDK5的底物包括p35，PAK1，Src，β-catenin，tau，neurofilament-H，neurofilament-M，synapsin1，APP，DARPP32，PP1-inhibitor和Rb。p35通过泛素-蛋白酶体途径迅速降解（T1/ 2 <20分钟）（1）。然而，CDK5激酶活性降低时，P35的稳定性增加时，可能是因为CDK5的作用减少了P35的Thr138位点磷酸化水平的缘故（2）。神经毒性损害后，蛋白酶使p35蛋白水解产生p25，导致CDK5长期被p25激活。研究发现，P25积聚在神经退行性疾病如阿尔茨海默病和肌萎缩性侧索硬化症（ALS）中（3,4）。

1. Dhavan, R. and Tsai, L.H. (2001) Nat Rev Mol Cell Biol 2, 749-59.
2. Patrick, G.N. et al. (1998) J Biol Chem 273, 24057-64.
3. Lee, M.S. et al. (2000) Nature 405, 360-4.
4. Kusakawa, G. et al. (2000) J Biol Chem 275, 17166-72.

原厂资料：

Specificity / Sensitivity

CDK5 (1H3) Mouse mAb recognizes endogenous levels of total CDK5 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the amino terminus of rat CDK5 protein.

Background

Cyclin-dependent kinases (CDKs) are serine/threonine kinases that are activated by cyclins and govern eukaryotic cell cycle progression. While CDK5 shares high sequence homology with its family members, it is thought mainly to function in postmitotic neurons to regulate the cytoarchitecture of these cells. Analogous to cyclins, the regulatory subunits p35 and p39 associate with and activate CDK5 despite the lack of sequence homology. CDK5 is ubiquitously expressed, with high levels of kinase activity detected primarily in the nervous system due to the narrow expression pattern of p35 and p39 in post-mitotic neurons. A large number of CDK5 substrates have been identified although no substrates have been specifically attributed to p35 or p39. Substrates of CDK5 include p35, PAK1, Src, β-catenin, tau, neurofilament-H, neurofilament-M, synapsin-1, APP, DARPP32, PP1-inhibitor, and Rb. p35 is rapidly degraded (T1/2 <20 min) by the ubiquitin-proteasome pathway (1). However, p35 stability increases as CDK5 kinase activity decreases, likely as a result of decreased phosphorylation of p35 at Thr138 by CDK5 (2). Proteolytic cleavage of p35 by calpain produces p25 upon neurotoxic insult, resulting in prolonged activation of CDK5 by p25. Research studies have shown accumulation of p25 in neurodegenerative diseases, such as Alzheimer's disease and amyotrophic lateral sclerosis (ALS) (3,4).

1. Dhavan, R. and Tsai, L.H. (2001) Nat Rev Mol Cell Biol 2, 749-59.
2. Patrick, G.N. et al. (1998) J Biol Chem 273, 24057-64.
3. Lee, M.S. et al. (2000) Nature 405, 360-4.
4. Kusakawa, G. et al. (2000) J Biol Chem 275, 17166-72.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.