SignalSilence? Sall4 siRNA II

• 产品编号：CST-12111S      品牌：CST       原厂货号：12111S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

Sall4 siRNA II可以抑制人和猴中Sall4 基因表达。来自Cell Signaling Technology (CST)的SignalSilence^® Sall4 siRNA II可以帮助研究者通过RNA干扰特异性地抑制Sall4的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST推荐使用100 nM SignalSilence^® Sall4 siRNA II 进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。每小瓶可供100次转染，每次转染量相当于在转染24孔板时，每孔总体积为300μl培养基中siRNA的终浓度为100nM。SALL基因家族成员编码发育过程中高表达的锌指转录因子（1）。Sall4与其他多潜能调控因子，例如Oct-4和Nanog，在发育的极早期就开始表达（2）。最近的研究提示Sall4作为主要调控子以一种转录调控反馈机制控制了其自身和Oct-4的表达以控制干细胞多潜能性和干细胞命运（2,3）。免疫组化实验指出Sall4是初级生殖细胞瘤和卵黄囊瘤的一个敏感且特异的诊断标志（4,5）。研究显示Sall4在急性髓细胞白血病（AML）中持续激活表达，是Wnt/β-catenin在该疾病中一个可能的效应因子（6）。除此以外，Sall4突变被发现可能涉及了人类畸形综合征，包括uane-radial ray 综合症 (Okihiro syndrome)和Acro-renal-ocular 综合症 (7)。

1. Sweetman, D. and Münsterberg, A. (2006) Dev Biol 293, 285-93.
2. Yang, J. et al. (2008) Proc Natl Acad Sci USA 105, 19756-61.
3. Yang, J. et al. (2010) PLoS One 5, e10766.
4. Mei, K. et al. (2009) Mod Pathol 22, 1628-36.
5. Miertus, J. et al. (2006) Hum Genet 119, 154-61.
6. Ma, Y. et al. (2006) Blood 108, 2726-35.
7. Al-Baradie, R. et al. (2002) Am J Hum Genet 71, 1195-9.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Sall4 siRNA II inhibits human and monkey Sall4 expression.

Description

SignalSilence® Sall4 siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Sall4 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM SignalSilence® Sall4 siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. 
 Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

Background

Members of the SALL gene family encode putative zinc finger transcription factors highly expressed during development (1). Sall4 is expressed very early in development with other pluripotency regulators, such as Oct-4 and Nanog (2). Recent studies suggest Sall4 works as a master regulator that controls its own expression and the expression of Oct-4 in a transcriptional regulation feedback loop governing stem cell pluripotency and stem cell fate (2,3). Immunohistochemical studies indicate that Sall4 is a sensitive and specific diagnostic marker for primary germ cell tumors and yolk sac tumors (4,5). Research studies have shown that Sall4 is constitutively expressed in acute myeloid leukemia (AML) and is a probable effector of the Wnt/β-catenin signaling pathway in this disease (6). In addition, mutations in Sall4 have been implicated in human malformation syndromes including Duane-radial ray syndrome (Okihiro syndrome) and Acro-renal-ocular syndrome (7).

1. Sweetman, D. and Münsterberg, A. (2006) Dev Biol 293, 285-93.
2. Yang, J. et al. (2008) Proc Natl Acad Sci USA 105, 19756-61.
3. Yang, J. et al. (2010) PLoS One 5, e10766.
4. Mei, K. et al. (2009) Mod Pathol 22, 1628-36.
5. Miertus, J. et al. (2006) Hum Genet 119, 154-61.
6. Ma, Y. et al. (2006) Blood 108, 2726-35.
7. Al-Baradie, R. et al. (2002) Am J Hum Genet 71, 1195-9.

注意事项：

Storage: Sall4 siRNA II is supplied in RNAse-free water. Aliquot
and store at -20ºC.