Aurora A (1F8) Mouse mAb

• 产品编号：CST-12100S      品牌：CST       原厂货号：12100S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Aurora A (1F8)Mouse mAb可以识别内源性总的Aurora A 蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人Aurora A蛋白的氨基端邻近残基序列一致。Aurora A (AIK)是一个细胞周期调控丝氨酸/苏氨酸蛋白酶，在多种癌细胞系表达上调（1-3）。位于Aurora A 的激酶激活区域288位苏氨酸被磷酸化后激活蛋白活性并可能导致蛋白在有丝分裂时通过蛋白酶体降解（4）。紧密联系的激酶Aurora B (AIM1)已被发现在多个有丝分裂事件中发挥作用（5），用siRNA敲除Aurora B会导致组蛋白H3磷酸化，异常的染色体对齐/隔离，改变survivin的细胞定位（6）。

1. Bischoff, J.R. et al. (1998) EMBO J 17, 3052-65.
2. Zhou, H. et al. (1998) Nat Genet 20, 189-93.
3. Sen, S. et al. (1997) Oncogene 14, 2195-200.
4. Walter, A.O. et al. (2000) Oncogene 19, 4906-16.
5. Kallio, M.J. et al. (2002) Curr Biol 12, 900-5.
6. Hauf, S. et al. (2003) J Cell Biol 161, 281-94.

原厂资料：

Specificity / Sensitivity

Aurora A (1F8) Mouse mAb recognizes endogenous levels of total Aurora A protein. 

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to human Aurora A protein.

Background

Aurora A (AIK) is a cell cycle-regulated Ser/Thr protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5), and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation, and altered survivin localization (6).

1. Bischoff, J.R. et al. (1998) EMBO J 17, 3052-65.
2. Zhou, H. et al. (1998) Nat Genet 20, 189-93.
3. Sen, S. et al. (1997) Oncogene 14, 2195-200.
4. Walter, A.O. et al. (2000) Oncogene 19, 4906-16.
5. Kallio, M.J. et al. (2002) Curr Biol 12, 900-5.
6. Hauf, S. et al. (2003) J Cell Biol 161, 281-94.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-mouse secondary antibodies must be used to
detect this antibody.
Recommended Antibody Dilutions:
Western blotting  1:1000
Immunofluorescence (IF-IC)  1:800
Flow Cytometry  1:1600