IFIT1 Antibody

• 产品编号：CST-12082S      品牌：Cell Signaling Technology       原厂货号：12082S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IFIT1抗体能识别内源性IFIT1总蛋白水平。此多克隆抗体通过用合成肽免疫动物制备，该合成肽是人IFIT1蛋白Ala194周围的残基。抗体通过蛋白A以及多肽亲和色谱法纯化。IFIT1（具有TPR1的干扰素诱导蛋白）属于IFIT蛋白质家族，该家族在人类中包括四个成员（IFIT1，IFIT2，IFIT3和 IFIT5），在小鼠中包括三个成员（IFIT1，IFIT2和IFIT3）（1）。IFIT1的表达受病毒感染产生的I型干扰素的诱导（2）。IFIT1是一种抗病毒蛋白能直接与有5'磷酸基团(PPP-RNA)的病毒RNA结合（2）。在人类中，病毒PPP-RNA结合IFIT1形成复合物与IFIT2和IFIT3一起隔离病毒PPP-RNA，并阻止复制（2）。IFIT1还能够与真核起始因子3 (eIF-3)结合抑制翻译（3）。

1. Ablasser, A. and Hornung, V. (2011) Nat Immunol 12, 588-90.
2. Pichlmair, A. et al. (2011) Nat Immunol 12, 624-30.
3. Guo, J. et al. (2000) EMBO J 19, 6891-9.

原厂资料：

Specificity / Sensitivity

IFIT1 Antibody recognizes endogenous levels of total IFIT1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala194 of human IFIT1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

IFIT1 (interferon-induced protein with tetratricopeptide repeats 1) belongs to the IFIT family of proteins, which consists of four members in humans (IFIT1, IFIT2, IFIT3, and IFIT5) and three members in mice (IFIT1, IFIT2, and IFIT3) (1). IFIT1 expression is induced by Type I Interferons resulting from viral infection (2). IFIT1 is an antiviral protein that directly binds viral RNA that has a 5’ triphosphate group (PPP-RNA) (2). In humans, the viral PPP-RNA bound IFIT1 forms a complex with IFIT2 and IFIT3 to sequester the viral PPP-RNA and prevent replication (2). IFIT1 has also been shown to inhibit translation by binding to the eukaryotic initiation factor-3 (eIF-3) (3).

1. Ablasser, A. and Hornung, V. (2011) Nat Immunol 12, 588-90.
2. Pichlmair, A. et al. (2011) Nat Immunol 12, 624-30.
3. Guo, J. et al. (2000) EMBO J 19, 6891-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.
Recommended Antibody Dilutions:
Western Blotting  1:1000
Immunoprecipitation  1:50