Ki-67 (D3B5) Rabbit mAb (Alexa Fluor? 647 Conjugate)

• 产品编号：CST-12075S      品牌：CST       原厂货号：12075S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Ki-67 (D3B5)Rabbit mAb（偶联Alexa Fluor® 647）可以识别内源性总的Ki-67蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人Ki-67蛋白的氨基端邻近残基序列一致。Cell Signaling Technology公司抗体偶联Alexa Fluor® 647荧光素染料并经人类细胞直接流式细胞仪免疫荧光分析内部测试。抗体预期和未偶联Alexa Fluor® 488的Ki-67 (D3B5)Rabbit mAb #9129显示同样的物种交叉反应。Ki-67，以其发现地命名（Kiel大学，德国），作为一个细胞核非组蛋白（1）通常在增殖的细胞中表达并且在休眠细胞中不表达（2）。Ki-67会让增殖细胞停滞在G1，S，G2和有丝分裂期，但是不会停滞在G0期。研究显示高表达的Ki-67与低乳腺癌存活率相关（3）。研究已经将Ki-67与其他的标记，作为乳腺癌和其他恶性疾病中的潜在的预后和预测标记（4）。

1. Gerdes, J. et al. (1983) Int J Cancer 31, 13-20.
2. Weigel, M.T. and Dowsett, M. (2010) Endocr Relat Cancer 17, R245-62.
3. Jones, R.L. et al. (2009) Breast Cancer Res Treat 116, 53-68.
4. Yerushalmi, R. et al. (2010) Lancet Oncol 11, 174-83.

原厂资料：

Specificity / Sensitivity

Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total Ki-67 protein.Ki-67 protein

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the amino terminus of human Ki-67 protein.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric and immunofluorescent analysis in human and rat cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Ki-67 (D3B5) Rabbit mAb #9129.

Background

Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4).

1. Gerdes, J. et al. (1983) Int J Cancer 31, 13-20.
2. Weigel, M.T. and Dowsett, M. (2010) Endocr Relat Cancer 17, R245-62.
3. Jones, R.L. et al. (2009) Breast Cancer Res Treat 116, 53-68.
4. Yerushalmi, R. et al. (2010) Lancet Oncol 11, 174-83.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the anti-
body. Protect from light. Do not freeze.
* Species cross-reactivity other than human is
determined by western blot using the unconjugated
antibody.
Recommended Antibody Dilutions:
Immunofluorescence (IF-IC)  1:50
Immunofluorescence (IF-F)  1:50
Flow Cytometry  1:50