MASTL (D3J4Y) Rabbit mAb

• 产品编号：CST-12069S      品牌：CST       原厂货号：12069S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MASTL (D3J4Y)Rabbit mAb可以识别内源性总的MASTL蛋白。.单克隆抗体由合成肽段免疫动物产生，该肽段与人MASTL蛋白的羧基端邻近残基序列一致。有丝分裂调控对所有真核细胞的正常生长，发育和维持都至关重要。研究证明失控的有丝分裂会导致基因组不稳定及癌症发生（1，2）。有丝分裂的一个调控因子，Greatwall 激酶（Gwl），最初在果蝇体内发现（3）。后续研究发现，基于序列同源性和功能，微管相关丝氨酸/苏氨酸激酶样蛋白（MASTL）是人体内的Gwl同源蛋白（4）。MASTL/Gwl的活性调控对于在正确的时机进入有丝分裂时非常重要的。研究发现磷酸化会激活Gwl（5）。人Gwl 194位和207位苏氨酸可以被cyclin B1-cdc2磷酸化而可能导致875位丝氨酸的自磷酸化（蟾蜍是883位丝氨酸）进而稳定这些激酶。激活的Gwl能分别磷酸化α-Endosulfine（ENSA）和cAMP-调控的磷酸蛋白19(ARPP19)的67和62位丝氨酸。磷酸化的ENSA和ARPP19会抑制蛋白磷酸酶2A(pp2A-B55)与B55亚基形成复合物，促进cyclin B1-cdc2的有丝分裂底物的完全磷酸化也促进细胞进入分裂期。Gwl失活后，PP2A-B55回复活力，将引发cyclin B1-cdc2的去磷酸化，细胞退出细胞周期（5,6,7）。

1. Eichhorn, P.J. et al. (2009) Biochim Biophys Acta 1795, 1-15.
2. Norbury, C. and Nurse, P. (1992) Annu Rev Biochem 61, 441-70.
3. Yu, J. et al. (2004) J Cell Biol 164, 487-92.
4. Voets, E. and Wolthuis, R.M. (2010) Cell Cycle 9, 3591-601.
5. Blake-Hodek, K.A. et al. (2012) Mol Cell Biol 32, 1337-53.
6. Vigneron, S. et al. (2011) Mol Cell Biol 31, 2262-75.
7. Lorca, T. and Castro, A. (2012) Oncogene 32, 537-543.

原厂资料：

Specificity / Sensitivity

MASTL (D3J4Y) Rabbit mAb recognizes endogenous levels of total MASTL protein. 

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MASTL protein.

Background

Mitotic control is important for normal growth, development, and maintenance of all eukaryotic cells. Research studies have demonstrated that inappropriate control of mitosis can lead to genomic instability and cancer (reviewed in 1,2). A regulator of mitosis, Greatwall kinase (Gwl), was first identified in Drosophila melanogaster (3). Subsequent studies showed that, based on sequence homology and function, microtubule-associated serine/threonine kinase-like (MASTL) is the human ortholog of Gwl (4). Regulation of MASTL/Gwl activation has been shown to be critical for the correct timing of mitosis. Research studies have shown that Gwl is activated by hyperphosphorylation (5). The phosphorylation of human Gwl at Thr194 and Thr207 by active cyclin B1-cdc2 leads to possible autophosphorylation at Ser875 (Ser883 in Xenopus), which stabilizes the kinase. Activated Gwl phosphorylates α-Endosulfine (ENSA) and cAMP-regulated phosphoprotein 19 (ARPP19) at Ser67 and Ser62, respectively. Phosphorylated ENSA and ARPP19 inhibit the activity of the B55 subunit-associated form of protein phosphatase 2A (PP2A-B55), allowing for complete phosphorylation of mitotic substrates by cyclin B1-cdc2 and mitotic entry. When Gwl is inactivated, PP2A-B55 reactivates, which leads to dephosphorylation of cyclin B1-cdc2 and mitotic exit (5,6, reviewed in 7).

1. Eichhorn, P.J. et al. (2009) Biochim Biophys Acta 1795, 1-15.
2. Norbury, C. and Nurse, P. (1992) Annu Rev Biochem 61, 441-70.
3. Yu, J. et al. (2004) J Cell Biol 164, 487-92.
4. Voets, E. and Wolthuis, R.M. (2010) Cell Cycle 9, 3591-601.
5. Blake-Hodek, K.A. et al. (2012) Mol Cell Biol 32, 1337-53.
6. Vigneron, S. et al. (2011) Mol Cell Biol 31, 2262-75.
7. Lorca, T. and Castro, A. (2012) Oncogene 32, 537-543.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.