mGluR2 Antibody

• 产品编号：CST-12056S      品牌：Cell Signaling Technology       原厂货号：12056S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

mGluR2 Antibody 能够检测内源性的mGluR2总蛋白。该抗体不会与mGluR3产生交叉反应。该多克隆抗体是由合成的小鼠源mGluR2蛋白丙氨酸(839位)肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。代谢型谷氨酸受体2（mGluR2）是哺乳动物脑中的神经递质谷氨酸的一种C类G蛋白偶联受体（GPCR）。不同于离子型受体，代谢型受体自己不形成离子通道孔，但与离子通道有间接联系（1）。当mGluR1和mGluR5激活磷脂酶C时，mGluR2、mGluR3、 mGluR4和mGluR6耦合到抑制性G蛋白Gα(i/o)并抑制腺苷酸环化酶（AC）的活性（1）。研究表明，mGluR2/3受体可能是精神分裂症治疗的潜在靶点（2）。此外，mGluR2与5HT2A5五羟色胺受体相互作用，在大脑中形成杂合复合物。这种复合物是致幻类药物的一个潜在药物靶点（3,4）。

原厂资料：

Specificity / Sensitivity

mGluR2 Antibody recognizes endogenous levels of total mGluR2 protein. This antibody does not cross-react with mGluR3.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala839 of mouse mGluR2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Metabotropic glutamate receptor 2 (mGluR2) is a class C G protein-coupled receptor for the neurotransmitter glutamate in the mammalian brain. Unlike ionotropic receptors, metabotropic receptors do not form an ion channel pore themselves but are indirectly linked to ion channels (1). While mGluR1 and mGluR5 activate phospholipase C, mGluR2, mGluR3, mGluR4, and mGluR6 are coupled to the inhibitory G protein Gα(i/o) and inhibit adenylyl cyclase (AC) activity (1). Research studies have suggested that mGluR2/3 receptors may be potential targets for the treatment of Schizophrenia (2). Furthermore, mGluR2 interacts with the 5HT2A serotonin receptor to form a hetero-complex in the brain. This complex is a potential pharmacological target for hallucinogenic drugs (3,4).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.
Recommended Antibody Dilutions:
Western blotting  1:1000
Immunoprecipitation  1:5