FoxK1 Antibody

• 产品编号：CST-12025S      品牌：CST       原厂货号：12025S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

FoxK1抗体可以识别内源性总的FoxK1蛋白。多抗由合成肽段免疫动物产生，该肽段与小鼠FoxK12蛋白Arg692的邻近氨基酸残疾序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。Forkhead box (Fox)蛋白家族是一类在进化上保守的转录因子，因其含有 winged螺旋的DNA结合域被称为Forkhead box (1)。人类中的Fox蛋白家族包含了40多个已知的成员，再分的19种亚家族蛋白参与调控多种发育进程中的高特异生物功能的基因转录(2)。通过广泛的蛋白芯片分析已发现多种影响Fox基因家族蛋白表达的突变，这与免疫异常、不育、演讲／语言障碍和肿瘤等多种人类缺陷相关(3,4)。FoxK1与FoxK2都属于Fox蛋白亚家族。FoxK1作为下游靶基因的转录抑制子发挥作用，靶基因包括FoxO4, Mef2, P21, PPGB, 和SM α-actin (5-7)。FoxK1有两个亚型：FoxK1-α和FoxK1-β。与α亚型相比，Β亚型有一个损伤的C末端。FoxK1的两个亚型在肌肉更新过程中表现为互补的表达模式。FoxK1-β主要表达在休眠的卫星细胞中（8），而在来源于激活的卫星细胞中的成肌细胞中FoxK1-α是主要的表达亚型（9）。两个FoxK1亚型共同调控发育和肌肉更新过程中的成肌干细胞增殖和分化（10）。

1. Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59.
2. Jackson, B.C. et al. (2010) Hum Genomics 4, 345-52.
3. Hannenhalli, S. and Kaestner, K.H. (2009) Nat Rev Genet 10, 233-40.
4. Benayoun, B.A. et al. (2011) Trends Genet 10, 224-32.
5. Hawke, T.J. et al. (2003) J Biol Chem 278, 4015-20.
6. Freddie, C.T. et al. (2007) Nucleic Acids Res 35, 5203-12.
7. Shi, X. et al. (2012) J Cell Sci , .
8. Garry, D.J. et al. (1997) Dev Biol 188, 280-94.
9. Yang, Q. et al. (1997) Mol Cell Biol 17, 5236-43.
10. Garry, D.J. et al. (2000) Proc Natl Acad Sci U S A 97, 5416-21.

原厂资料：

Specificity / Sensitivity

FoxK1 Antibody recognizes endogenous levels of total FoxK1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg692 of mouse FoxK1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors defined by the presence of a winged helix DNA binding domain called a Forkhead box (1). In humans, there are over 40 known Fox protein family members, divided into 19 subfamilies, which have evolved to regulate gene transcription in diverse and highly specialized biological contexts throughout development (2). Mutations that disrupt the expression of Fox gene family members have consequently been implicated in a broad array of human disorders, including immunological dysfunction, infertility, speech/language disorders, and cancer (3,4).FoxK1 belongs to a subfamily of Fox proteins along with FoxK2. FoxK1 functions as a transcriptional repressor of downstream target genes including FoxO4, Mef2, P21, PPGB, and SM α-actin (5-7). There are two isoforms of FoxK1: FoxK1-α and FoxK1-β. The β isoform has a truncated C terminus compared to the α isoform. The two isoforms of FoxK1 show reciprocal expression patterns during muscle regeneration. FoxK1-β is expressed mainly in quiescent satellite cells (8), whereas FoxK1-α is the dominant isoform in proliferating myoblasts from activated satellite cells (9). Both isoforms of FoxK1 jointly regulate proliferation and differentiation of myogenic stem cells during development and muscle regeneration (10).

1. Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59.
2. Jackson, B.C. et al. (2010) Hum Genomics 4, 345-52.
3. Hannenhalli, S. and Kaestner, K.H. (2009) Nat Rev Genet 10, 233-40.
4. Benayoun, B.A. et al. (2011) Trends Genet 10, 224-32.
5. Hawke, T.J. et al. (2003) J Biol Chem 278, 4015-20.
6. Freddie, C.T. et al. (2007) Nucleic Acids Res 35, 5203-12.
7. Shi, X. et al. (2012) J Cell Sci , .
8. Garry, D.J. et al. (1997) Dev Biol 188, 280-94.
9. Yang, Q. et al. (1997) Mol Cell Biol 17, 5236-43.
10. Garry, D.J. et al. (2000) Proc Natl Acad Sci U S A 97, 5416-21.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.