Mouse?His6Interleukin-25/IL-17E (mHis6IL-25)

• 产品编号：CST-11996SC      品牌：CST       原厂货号：11996SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > IL-17 Family 白细胞介素-17超家族
• 应用分类：

描述：

重组小鼠 _His6IL-25 (m_His6IL-25) Val17-Ala169 (Accession #NP_542767) 通过Cell Signaling Technology公司的人源293细胞表达。重组的N-端_His6标签mIL-25蛋白，其分子量据推算为20,051 Da，DTT-还原处理后的蛋白在24-31kDa之间，未还原的胱氨酸连接的同源二聚体在46-50kDa之间。其在SDS-PAGE胶中的低迁移性和异质性与糖基化有关。重组小鼠_His6IL-25蛋白的氨基酸末端通过氨基酸测序得到。6 μg 还原 (+) 和 非还原的(-) 重组小鼠_His6IL-25通过SDS-PAGE检测，纯度大于97%，所有批次的纯度均高于97%。重组小鼠_His6IL-25 的生物活性是通过小鼠脾细胞和HT-29细胞的因子分泌试验确定的。每个样品的ED50在0.5-5 pg/ml之间（小鼠脾细胞）和0.25-1.5 ng/mL之间（HT-29细胞）。每微克_His6IL-25内毒素含量少于0.01 ng。

有载体：每微克_His6IL-25溶解在包含 20 μg BSA的20 mM PBS （pH 7.2）溶液中，并通过 0.22 μm 滤膜过滤后冻干。

无载体：每微克_His6IL-25溶解在20 mM PBS （pH 7.2）溶液中，，并通过 0.22 μm 滤膜过滤后冻干。IL-25，又称IL-17E，是IL-17超家族细胞因子成员之一。IL-25在上皮细胞，CD4⁺T细胞，肥大细胞以及嗜酸性粒细胞中表达(1)。多种细胞类型都可以对IL-25产生应答，如T细胞，巨噬细胞和上皮细胞(1)。IL-25受体是由IL-17RA和IL-17RB组成的异源二聚体(1,2)。IL-25能够通过诱导IL-5、IL-4和IL-13促进Th2型免疫应答，同时可能有助于过敏性炎症和哮喘的发生(1-3)。IL-25同样能够促进Th9细胞的活化以及诱导乳腺癌细胞的凋亡(4,5)。

原厂资料：

Source / Purification

Recombinant mouse _His6IL-25 (m_His6IL-25) Val17-Ala169 (Accession #NP_542767) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant N-terminally _His6-tagged mIL-25 has a calculated MW of 20,051 Da. DTT-reduced protein migrates as a 24-31 kDa polypeptide. The nonreduced cystine-linked homodimer migrates as a 46-50 kDa protein. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino terminus of recombinant m_His6IL-25 was verified by amino acid sequencing.

Purity

>97% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant m_His6IL-25. All lots are greater than 97% pure.

Bioactivity

The bioactivity of recombinant m_His6IL-25 was determined in mouse splenocyte and HT-29 cell cytokine secretion assays. The ED50 of each lot is between 0.5-1.5 ng/ml in mouse splenocyte cells and 0.25-1.5 ng/mL in HT-29 cells.

Endotoxin

Less than 0.01 ng endotoxin/1 μg m_His6IL-25.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of m_His6IL-25 in 20 mM Tris, pH 7.2 containing 20 μg BSA per 1 μg m_His6IL-25. Carrier free: Lyophilized from a 0.22 μm filtered solution of m_His6IL-25 in 20 mM Tris, pH 7.2.

Background

IL-25, also known as IL-17E, is a member of the IL-17 superfamily of cytokines. IL-25 is expressed in epithelial cells, CD4+ T cells, mast cells, and eosinophils (1). Many cell types are responsive to IL-25, including T cells, macrophages, and epithelial cells (1). The receptor for IL-25 consists of a heterodimer of IL-17RA and IL-17RB (1,2). IL-25 promotes Th2 type immune responses by induction of IL-5, IL-4, and IL-13 and may contribute to allergic inflammation and asthma (1-3). IL-25 has also been shown in research to promote Th9 cell activation and induce apoptosis in breast cancer cells (4,5).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.