Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb

• 产品编号：CST-11992S      品牌：CST       原厂货号：11992S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

当丝氨酸（159 和163位）（人肽段）磷酸化时，Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb识别内源性的总MARCKS蛋白。该单克隆抗体通过用合成磷酸肽免疫动物制备，该合成肽与人MARCKS蛋白丝氨酸（159位和163位）附近的残基一样。豆寇酰化富含丙氨酸C-激酶底物（MARCKS）是一种重要的PKC底物，在多种细胞类型中表达。 MARCKS与细胞活力，细胞粘附，吞噬，膜运输和有丝分裂有关（1）。PKC响应生长因子和氧化应激磷酸化MARCKS的丝氨酸（159，163，167和170位），调节钙/钙调蛋白结合和MARCKS丝状（F）-肌动蛋白交联反应活性（2-4）。PKC磷酸化作用导致MARCKS从细胞膜到细胞质迁移(5)。

1. Ramsden, J.J. (2000) Int J Biochem Cell Biol 32, 475-9.
2. Heemskerk, F.M. et al. (1993) Biochem Biophys Res Commun 190, 236-41.
3. Graff, J.M. et al. (1989) J Biol Chem 264, 21818-23.
4. Hartwig, J.H. et al. (1992) Nature 356, 618-22.
5. Thelen, M. et al. (1991) Nature 351, 320-2.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey, Chicken, Xenopus, Zebrafish, Bovine, Pig

Specificity / Sensitivity

Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb recognizes endogenous levels of MARCKS protein when phosphorylated at Ser159 and Ser163 (human residues).

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser159 and Ser163 of human MARCKS protein.

Background

Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a major PKC substrate expressed in many cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic, and mitogenesis (1). PKC phosphorylates Ser159, 163, 167, and 170 of MARCKS in response to growth factors and oxidative stress. Phosphorylation at these sites regulates the calcium/calmodulin binding and filamentous (F)-actin cross-linking activities of MARCKS (2-4). Phosphorylation by PKC also results in translocation of MARCKS from the plasma membrane to the cytoplasm (5).

1. Ramsden, J.J. (2000) Int J Biochem Cell Biol 32, 475-9.
2. Heemskerk, F.M. et al. (1993) Biochem Biophys Res Commun 190, 236-41.
3. Graff, J.M. et al. (1989) J Biol Chem 264, 21818-23.
4. Hartwig, J.H. et al. (1992) Nature 356, 618-22.
5. Thelen, M. et al. (1991) Nature 351, 320-2.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.