BRM (D9E8B) XP? Rabbit mAb

• 产品编号：CST-11966P      品牌：Cell Signaling Technology       原厂货号：11966P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

BRM (D9E8B) XP® Rabbit mAb兔单抗识别内源性BRM总蛋白水平。该抗体不与BRG1蛋白发生交叉反应。

该该单克隆抗体是采用合成的与人源BRM蛋白Gly264残基周围序列相对应的肽段免疫动物而生产的。

ATP依赖的染色质重塑复合物，在调节如转录、DNA的复制和修复等细胞核反应起着重要作用(1,2)。SWI/SNF染色质重塑复合物由10多个亚基组成，BRM或BRG1任一单分子作为ATP酶催化亚基，但是两者不能同时存在。BRM/BRG1能够阻断组蛋白与DNA的结合，改变染色质内重要的辅助调控元件(2-5)。含有BRM/BRG1的SWI/SNF复合物招募一些转录因子靶向启动子，如细胞核受体、P53、RB和BRCA1,从而调节细胞生长、细胞周期、细胞分化等过程(1,6-9)。BRM及BRG1也被认为是肿瘤抑制基因，在几种癌症细胞中，两者的表达水平严重降低(10-13)。

1. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
2. Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73.
3. Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11.
4. Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81.
5. Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17.
6. Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32.
7. Morettini, S. et al. (2008) Front Biosci 13, 5522-32.
8. Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6.
9. Simone, C. (2006) J Cell Physiol 207, 309-14.
10. Yamamichi, N. et al. (2005) Oncogene 24, 5471-81.
11. Reisman, D.N. et al. (2002) Oncogene 21, 1196-207.
12. Shen, H. et al. (2008) Cancer Res 68, 10154-62.
13. Weissman, B. and Knudsen, K.E. (2009) Cancer Res 69, 8223-30.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Dog

Specificity / Sensitivity

BRM (D9E8B) XP® Rabbit mAb recognizes endogenous levels of total BRM protein. This antibody does not cross-react with BRG1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly264 of human BRM protein

Background

ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes, such as gene expression, DNA replication, and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits with a single molecule of the ATPase catalytic subunit BRM or BRG1, but not both. The activities of these two subunits drive the disruption of histone-DNA contacts that lead to changes in accessibility of crucial regulatory elements within chromatin (2-5). The BRM/BRG1 containing SWI/SNF complexes are recruited to target promoters by transcription factors, such as nuclear receptors, p53, RB, and BRCA1 to regulate gene activation, cell growth, the cell cycle, and differentiation processes (1,6-9). BRM and BRG1 are also considered to be tumor suppressors and their expression levels are severely reduced in several cancer cell lines (10-13).

1. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
2. Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73.
3. Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11.
4. Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81.
5. Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17.
6. Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32.
7. Morettini, S. et al. (2008) Front Biosci 13, 5522-32.
8. Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6.
9. Simone, C. (2006) J Cell Physiol 207, 309-14.
10. Yamamichi, N. et al. (2005) Oncogene 24, 5471-81.
11. Reisman, D.N. et al. (2002) Oncogene 21, 1196-207.
12. Shen, H. et al. (2008) Cancer Res 68, 10154-62.
13. Weissman, B. and Knudsen, K.E. (2009) Cancer Res 69, 8223-30.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
**Anti-rabbit secondary antibodies must be used to
detect this antibody.