Human IL-4 Neutralizing (D20H1) Rabbit mAb

• 产品编号：CST-11964S      品牌：CST       原厂货号：11964S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

人 IL-4 Neutralizing (D20H1) Rabbit mAb（中和兔单抗） 能够结合人源IL-4(hIL-4)并且中和其在TF-1细胞增殖过程中的活性。这种抗体不与鼠源IL-4，人源IL-13以及鼠源IL-13发生交叉反应。

此单克隆抗体是通过重组hIL-4蛋白免疫动物制备。
IL-4可由T 细胞、NK T 细胞、γδ 细胞和肥大细胞产生(1)，靶细胞包括B 细胞、T 细胞和巨噬细胞(1)。IL-4 诱导幼稚辅助性T细胞极化为TH2表型。IL-4也能促进B细胞增殖、抗体类别转换、以及产生其它TH2细胞因子，如IL-5和IL-9。IL-4诱导TH2的极化对病原菌体液免疫的发生有重要作用(1)，同时也参与到敏感症和哮喘的发病过程中(2)。IL-4 结合到两个不同的受体上, I型受体和II型受体。I型受体是异源二聚体，包含IL-4Rα 链和普遍存在的 γ链-γc (3,4)。II型受体, 与IL-13受体共享, 是IL-4Rα 和 IL-13Rα1的异源二聚体。通过I型受体进行的信号传递能激活 Jak1/Stat6、Jak3 和 PI3K/Akt通路 (3)。II型受体能激活 Jak1/Stat6和Tyk2/Stat3通路(3)。

原厂资料：

Specificity / Sensitivity

Human IL-4 Neutralizing (D20H1) Rabbit mAb binds to human IL-4 (hIL-4) and neutralizes its effects in a TF-1 cell proliferation assay. This antibody does not cross-react with mouse IL-4, human IL-13, or mouse IL-13.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant hIL-4 protein.

Description

Neutralizing antibodies can be used to inhibit normal biological function through their binding to biological molecules. These reagents can be used to determine the effects that a particular molecule has in biological systems. Human IL-4 Neutralizing (D20H1) Rabbit mAb has been shown to neutralize the proliferation of TF-1 cells in vitro with an ND50 in the range of 3-19 ng/ml.

Background

Interleukin-4 (IL-4) is produced by T cells, NK T cells, γδ cells, and mast cells (1). Target cells include B cells, T cells, and macrophages (1). IL-4 induces differentiation of naive T cells into the Th2 phenotype. IL-4 also promotes B cell proliferation, antibody isotype switching, and expression of other Th2 cytokines including IL-5 and IL-9. IL-4-induced Th2 polarization is important in developing humoral immunity against extracellular pathogens (1) and is involved in the development of allergy and asthma (2). IL-4 binds to two distinct receptors, the type I receptor and type II receptor. The type I receptor is a heterodimer consisting of IL-4Rα chain and the common gamma chain, γc (3,4). The type II receptor, which is shared with IL-13, is a heterodimer of IL-4Rα and IL-13Rα1. Signaling initiated via type I receptor results in the activation of Jak1/Stat6, Jak3, and the PI3K/Akt pathways (3). The type II receptor activates the Jak1/Stat6 and the Tyk2/Stat3 pathways (3).

注意事项：

Formulation: Lyophilized from a 0.2 µm filtered solution in
10 mM HEPES with trehalose.
Reconstitution: Add sterile 10 mM HEPES pH 7.0 to a final
concentration of greater than 50 μg/ml. Solubilize for 20 min at
room temperature with occasional gentle vortexing.
Storage: Store lyophilized material at -20ºC. After reconstitu-
tion, recommended storage at 4ºC for 1 month or -20ºC for 6
months. Avoid repeated freeze/thawing.