DLST (D22B1)?XP??Rabbit mAb

• 产品编号：CST-11954P      品牌：Cell Signaling Technology       原厂货号：11954P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DLST (D22B1) XP® Rabbit mAb 兔单抗能够检测内源性DLST总蛋白水平。

该单克隆抗体是由合成的人源DLST蛋白脯氨酸(188位)肽段免疫动物而制备的。

α酮戊二酸脱氢酶复体物是线粒体柠檬酸循环中的限速酶（1）。二氢硫辛酰胺－琥珀酰转移酶(DLST)是该复合物中的关键亚基（2）。下调表达DLST会增加活性氧的产量，这表明了该基因在氧化应激中所发挥的作用（2）。研究表明，小鼠中DLST缺失与线粒体氧化应激的增加有关，并且会出现阿尔茨海默病的病理现象（2）。

1. Ohta, S. and Ohsawa, I. (2006) J Alzheimers Dis 9, 155-66.
2. Dumont, M. et al. (2009) Free Radic Biol Med 47, 1019-27.

原厂资料：

Specificity / Sensitivity

DLST (D22B1) XP® Rabbit mAb recognizes endogenous levels of total DLST protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro188 of human DLST protein.

Background

α-ketoglutarate dehydrogenase complex is a rate-regulating enzyme in the Krebs Cycle (1). Dihydrolipoamide succinyltransferase (DLST) is a key subunit in this complex (2). Reduction of DLST increases reactive oxygen species production, suggesting its role in oxidative stress (2). Research has shown that deficiency of DLST in mice is linked to increased oxidative stress in mitochondria, a process that may be involved in the pathogenesis of Alzheimer's disease (2).

1. Ohta, S. and Ohsawa, I. (2006) J Alzheimers Dis 9, 155-66.
2. Dumont, M. et al. (2009) Free Radic Biol Med 47, 1019-27.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.