Human TL1A/TNFSF15 (hTL1A)

• 产品编号：CST-11946LC      品牌：CST       原厂货号：11946LC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > IL-1 Family 白细胞介素-1超家族
• 应用分类：

描述：

重组人TL1A Leu72-Leu251 (Accession #NP_095150)由CST公司在大肠杆菌中进行表达。

重组hTL1A的分子量为20,047Da。DTT还原蛋白接近21 kDa的多肽的迁移位置。非还原蛋白分为21kDa的单体和38kDa的胱氨酸联同型二聚体。重组hTL1A预期的氨基末端通过氨基酸测序进行了验证

重组hTL1A的分子量为20,047Da。DTT还原蛋白接近21 kDa的多肽的迁移位置。非还原蛋白分为21kDa的单体和38kDa的胱氨酸联同型二聚体。重组hTL1A预期的氨基末端通过氨基酸测序进行了验证

有载体：将hTL1A溶液（20mM Tris，pH7.2，每1 μg hTL1A中含20 μg BSA）经0.22μm滤膜过滤并冻干。

无载体：将hTL1A溶液（20mM Tris，pH7.2）经0.22μm滤膜过滤并冻干。

TL1A (TNFSF15)是肿瘤坏死因子超家族成员之一，是TL1/VEGI基因的剪接变体（1）。内皮细胞、单核细胞、巨噬细胞和树突细胞都能表达TL1A，TL1A在促炎细胞因子、微生物和AMPK活化的作用下能上调（1-4）。TL1A通过其受体DR3能活化NF-κB和JNK通路（1,5）。TL1A可能作为T细胞活化的协同刺激信号，尤其是调节Th17细胞发育和增殖（1,2,6）。小鼠模型显示TL1A是炎性肠病相关炎症和发病机制的“驱动器”（7,8）。

原厂资料：

Source / Purification

Recombinant Human TL1A Leu72-Leu251 (Accession #NP_095150) was expressed in E.coli at Cell Signaling Technology. 

Molecular Characterization

Recombinant hTL1A has a calculated MW of 20,047Da. DTT-reduced protein migrates as a 21 kDa polypeptide. The nonreduced protein migrates as a 21 kDa monomer and 38 kDa cystine-linked homodimer. The expected amino terminus of recombinant hTL1A was verified by amino acid sequencing.

Purity

>95% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hTL1A. All lots are greater than 95% pure.

Bioactivity

The bioactivity of hTL1A was determined in a TF-1 cell viability assay. The ED50 of each lot is between 2-15 ng/ml. 

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of hTL1A in 20 mM Tris, pH 7.2 containing 20 μg BSA per 1 μg hTL1A. Carrier free: Lyophilized from a 0.22 μm filtered solution of hTL1A in 20 mM Tris, pH 7.2.

Background

TL1A (TNFSF15), a member of the TNF superfamily of proteins, is a splice variant of the TL1/VEGI gene (1). Endothelial cells, monocytes, macrophages, and dendritic cells express TL1A, which is upregulated by proinflammatory cytokines, microorganisms, and AMPK activation (1-4). TL1A activates the NF-κB and JNK pathways through its receptor, DR3 (1,5). TL1A may function as a costimulatory signal for T cell activation, specifically regulating Th17 cell development and proliferation (1,2,6). Mouse models suggest a role for TL1A as a driver for the inflammation and pathogenesis associated with inflammatory bowel disease (7,8).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.