PC1/3 Antibody

• 产品编号：CST-11914S      品牌：CST       原厂货号：11914S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PC1/3 Antibody兔多抗能够检测内源性的PC1/3总蛋白。该多克隆抗体是由合成的人源PC1/3蛋白精氨酸(364位)肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。前蛋白转化酶 （PCs）是一种在分泌途径内通过蛋白水解裂解酶激活前体蛋白的酶。PCs包含几种酶，碱性氨基酸特异性蛋白酶（弗林蛋白酶，PC1/3，PC2，PC4，PACE4，PC5/6和PC7），以及非碱性氨基酸转化酶 （S1P和PC9）（1）。 PCs有一个共同的结构，其中包括一个N-末端的信号肽用于靶向到分泌途径；一个被认为是分子内的分子伴侣的亲域；一个含有的活性位点的催化结构域；一个通过调节稳定性，钙离子和pH值依赖的方式作用于酶的整体折叠的P-域；以及一个与膜相互作用的C-末端结构域（2）。PCs以组织和底物特异的方式在大脑和外周产生了一系列的生物活性肽和蛋白质的前体（3）。对PCSK1基因（编码PC1/3）的研究表明，该基因的突变会使PC1/3表达或酶活性下降，促进肥胖（4）。

1. Scamuffa, N. et al. (2006) FASEB J 20, 1954-63.
2. Fugère, M. and Day, R. (2005) Trends Pharmacol Sci 26, 294-301.
3. Seidah, N.G. and Chrétien, M. (1999) Brain Res 848, 45-62.
4. Creemers, J.W. et al. (2012) Diabetes 61, 383-90.

原厂资料：

Specificity / Sensitivity

PC1/3 Antibody recognizes endogenous levels of total PC1/3 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg364 of human PC1/3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The proprotein convertases (PCs) are enzymes that activate precursor proteins through proteolytic cleavage within the secretory pathway. PCs comprise several enzymes that are basic amino acid-specific proteinases (furin, PC1/3, PC2, PC4, PACE4, PC5/6, and PC7), as well as nonbasic amino acid convertases (S1P and PC9) (1). PCs have a common structure that includes an N-terminal signal peptide for secretory pathway targeting; a pro-domain that is thought to act as an intramolecular chaperone; a catalytic domain containing the active site; a P-domain that contributes to the overall folding of the enzyme by regulating stability, calcium-, and pH-dependence; and a C-terminal domain that interacts with the membrane (2). PCs act in a tissue- and substrate-specific fashion to generate an array of bioactive peptides and proteins from precursors, both in the brain and the periphery (3). Research studies examining the PCSK1 gene (encoding PC1/3) suggest that mutations that cause impaired PC1/3 expression or enzymatic activity contribute to obesity (4).

1. Scamuffa, N. et al. (2006) FASEB J 20, 1954-63.
2. Fugère, M. and Day, R. (2005) Trends Pharmacol Sci 26, 294-301.
3. Seidah, N.G. and Chrétien, M. (1999) Brain Res 848, 45-62.
4. Creemers, J.W. et al. (2012) Diabetes 61, 383-90.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to detect this antibody.
Recommended Antibody Dilutions:
Western blotting 1:1000