NFI-C Antibody

• 产品编号：CST-11911S      品牌：CST       原厂货号：11911S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NFI-C Antibody能够检测内源性的NFI-C总蛋白水平。该抗体还能够检测过表达的NFI-B蛋白。该多克隆抗体是采用合成的与人源NFI-C蛋白Ala263残基周围序列相对应的肽段免疫动物而生产的。并经过Protein A和多肽亲和层析纯化。

NFI-C属于能够调控病毒DNA复制和多种基因表达的位点特异性转录因子的nuclear factor I (NFI)家族。在脊椎动物中，NFI家族由四种成员组成：NFI-A, NFI-B, NFI-C, 以及 NFI-X。这四个成员对于小鼠和人内多个器官系统的发育调控是至关重要的（3）。NFI-C在多种组织中均有表达，它能够调控TGF-β以来的牙齿发育和毛囊周期（3-5）。研究发现NFI-C能直接阻遏FoxF1的转录并抑制乳腺癌细胞的迁移和侵袭（6）。

1. Gronostajski, R.M. (2000) Gene 249, 31-45.
2. Liang, B. et al. (2012) J Virol 86, 3264-75.
3. Steele-Perkins, G. et al. (2003) Mol Cell Biol 23, 1075-84.
4. Lee, D.S. et al. (2009) J Biol Chem 284, 17293-303.
5. Plasari, G. et al. (2010) J Biol Chem 285, 34115-25.
6. Nilsson, J. et al. (2010) Cancer Res 70, 2020-9.

原厂资料：

Specificity / Sensitivity

NFI-C Antibody recognizes endogenous levels of total NFI-C protein. This antibody also recognizes overexpressed NFI-B protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala263 of human NFI-C protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

NFI-C belongs to the nuclear factor I (NFI) family of site-specific transcription factors that regulate viral DNA replication and expression of various genes (1,2). The NFI family is composed of four members in vertebrates: NFI-A, NFI-B, NFI-C, and NFI-X, all of which are critical in the development of multiple organ systems in mice and humans (3). NFI-C is expressed in various tissues and regulates TGF-β dependent tooth development and hair follicle cycling (3-5). Research studies have shown that NFI-C directly represses FoxF1 transcription and suppresses the motility and invasiveness of breast cancer cells (6).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to detect this antibody.
Recommended Antibody Dilutions:
Western blotting 1:1000
Immunoprecipitation 1:50