Toll-like Receptor 8 (D3Z6J) Rabbit mAb

• 产品编号：CST-11886S      品牌：CST       原厂货号：11886S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TLR8 (D3Z6J) Rabbit mAb抗体能识别内源性TLR8 总蛋白水平。此抗体与来源不明的30kDa和37kDa的蛋白有交叉反应。此单克隆抗体通过用合成肽免疫动物制备，该合成肽是人TLR8蛋白Pro141周围的残基。

Toll样受体（TLR）家族成员是对果蝇中密切相关的一系列Toll样受体的命名，它们在先天免疫反应中发挥着举足轻重的作用（1-4）。TLRs能识别各种病原体中的保守基序和调解防御反应（5-7）。TLR通路的触发往往导致NF-κB的活化及随后的免疫和炎症基因的调控（4）。TLRs和白介素1受体家族成员共享一个约200个氨基酸的保守区域，称为Toll/IL-1受体（TIR）结构域（1）。激活后，TLRs与许多细胞质（包含TIR结构域）的接头蛋白相关，包括髓样分化因子88（MyD88）、MyD88样接头蛋白/TIR结构域接头蛋白（MAL/TIRAP）、Toll受体相关的干扰素活化剂（TRIF）和Toll受体相关的分子（TRAM）（8-10）。这种关联导致IRAK1和IRAK4的补充和活化，能与TRAF6形成复合物激活TAK1和IKK（8,11-14）。IKK的活化能导致IκB的降解，通常情况下，IκB使NF-κB以失活状态存在于细胞质中。

TLR8是一种细胞内的TLR，位于内质网、内涵体、溶酶体和内吞溶酶体（4）。它能被单链病毒RNA和合成的咪唑喹啉类化合物（包括R-848）激活（5）。TLR8的表达在肺和髓系细胞中最高（6,7）。另外，TLR8可由单核细胞样白血病THP-1细胞（受TPA诱导向单核系方向分化）中的IFN-γ引起表达上调（7）。

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原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

TLR8 (D3Z6J) Rabbit mAb recognizes endogenous levels of total TLR8 protein. This antibody cross-reacts with a 30 kDa protein and a 37 kDa protein of unknown origin.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro141 of human TLR8 protein.

Background

Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm.

Background

Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to detect this antibody.
Recommended Antibody Dilutions:
Western blotting 1:1000