Ki-67 (D3B5) Rabbit mAb?(Alexa Fluor??488 Conjugate)

• 产品编号：CST-11882S      品牌：CST       原厂货号：11882S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

protein.Ki-67 (D3B5) Rabbit mAb (偶联Alexa Fluor® 488)可以识别内源性总的Ki-67蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人Ki-67蛋白的氨基端一致。Cell Signaling Technology公司抗体偶联Alexa Fluor® 488荧光素染料并经人类细胞直接流式细胞仪免疫荧光分析内部测试。抗体预期和未偶联Alexa Fluor® 488的Ki-67 (D3B5)Rabbit mAb #9129显示同样的物种交叉反应.

原厂资料：

Specificity / Sensitivity

Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total Ki-67 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Ki-67 protein.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric and immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Ki-67 (D3B5) Rabbit mAb #9129.

Background

Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4)

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the
antibodies. Protect from light. Do not freeze.
* Species cross-reactivity other than human and rat is determined by western blot using the unconjugated antibody.
Recommended Antibody Dilutions:
Immunofluorescence (IF-IC) 1:50
Immunofluorescence (IF-F) 1:50
Flow Cytometry 1:50