Phospho-HS1 (Tyr397) (D12C1) XP? Rabbit mAb (PE Conjugate)

• 产品编号：CST-11880S      品牌：CST       原厂货号：11880S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (PE偶联)兔单抗只能检测Tyr397位点发生磷酸化时的内源性HS1蛋白水平。

此单克隆抗体通过合成鼠源HS1蛋白Tyr405位点周围的肽段免疫动物制备。这个位点与人源HS1蛋白的Tyr397相对应。

这种Cell Signaling Technology的抗体与藻红蛋白(PE)相偶联，并且通过对人源细胞的流式细胞分析来进行内部检测。此抗体与未偶联的Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb #8714一样，具有同物种之间的交叉反应性。

HS1 (HCLS1, LckBP1, p75)是一种蛋白激酶底物，它只在具有造血功能的组织和细胞中表达(1,2)。HS1 包含了四个皮肌动蛋白重复和一个单独的SH3 域(2)。在免疫受体的激活后，胞内蛋白被磷酸化,从而促进了HS1被招募到免疫突触处(3-5)。HS1的磷酸化是调控肌动蛋白动力不可缺少的，同时也为其他的信号分子如Vav1和 PLCγ1 提供了结合位点(6)。HS1也在血小板激活中发挥了重要的作用(7)。

在免疫受体刺激和凝血酶介导的血小板刺激下，HS1能够快速的被Syk和/或 Lyn激酶在Tyr397位点处磷酸化. 此磷酸化是细胞骨架重排和信号复合体形成过程中重要的一步(6-10)。

1. Kitamura, D. et al. (1989) Nucleic Acids Res 17, 9367-79.
2. Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
3. Suzuki, H. et al. (1997) J Immunol 159, 5881-8.
4. Hata, D. et al. (1994) Immunol Lett 40, 65-71.
5. Yamanashi, Y. et al. (1993) Proc Natl Acad Sci USA 90, 3631-5.
6. Gomez, T.S. et al. (2006) Immunity 24, 741-52.
7. Kahner, B.N. et al. (2007) Blood 110, 2449-56.
8. Yamanashi, Y. et al. (1997) J Exp Med 185, 1387-92.
9. Hao, J.J. et al. (2004) J Biol Chem 279, 33413-20.
10. Brunati, A.M. et al. (2005) J Biol Chem 280, 21029-35.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of HS1 protein only when phosphorylated at Tyr397.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr405 of mouse HS1 protein. This site corresponds to Tyr397 of human HS1 protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAb #8714.

Background

HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7).

1. Kitamura, D. et al. (1989) Nucleic Acids Res 17, 9367-79.
2. Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
3. Suzuki, H. et al. (1997) J Immunol 159, 5881-8.
4. Hata, D. et al. (1994) Immunol Lett 40, 65-71.
5. Yamanashi, Y. et al. (1993) Proc Natl Acad Sci USA 90, 3631-5.
6. Gomez, T.S. et al. (2006) Immunity 24, 741-52.
7. Kahner, B.N. et al. (2007) Blood 110, 2449-56.
8. Yamanashi, Y. et al. (1997) J Exp Med 185, 1387-92.
9. Hao, J.J. et al. (2004) J Biol Chem 279, 33413-20.
10. Brunati, A.M. et al. (2005) J Biol Chem 280, 21029-35.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2mg/ml BSA. Store at 4°C.

Do not aliquot the antibody. Pritect from light .Do not freeze !

Recommended Antibody Dilutions:
Flow Cytometry 1:50