Phospho-GIT2 (Tyr392) (D8N9A) Rabbit mAb

• 产品编号：CST-11873S      品牌：CST       原厂货号：11873S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-GIT2 (Tyr392) (D8N9A) Rabbit mAb可以识别392位酪氨酸磷酸化的GIT2蛋白。抗体与其他的酪氨酸磷酸化的蛋白由微弱的交叉反应。单克隆抗体由合成肽段免疫动物产生，该肽段与人GIT2的Tyr392位丝氨酸邻近残基序列一致。

G蛋白偶联受体（GPCR）激酶相互作用蛋白1和2(GIT1和GIT2)是高度保守，广泛表达的折叠蛋白，涉及细胞信号对黏着斑连接和细胞骨架活性。GIT蛋白含有多个相互作用的结构域能够与小GTPase（包括ARF，Rsc和cdc42），激酶（例如PAK和MEK），Rho家族GEF Pix和黏着斑蛋白paxillin（1）。GIT1和GIT2共享许多相同的属性，但至少有十个不同的组织特异性剪接变异体。GIT2在抑制黏着斑翻转和细胞膜突出物方面发挥作用（2,3）。GIT2的黏着斑定位和paxillin结合是通过FAK和/或Src磷酸化它的一个或多个酪氨酸位点（Tyr286, Tyr392, Tyr592）而被调控的（4,5,6）。

1. Hoefen, R.J. and Berk, B.C. (2006) J Cell Sci 119, 1469-75.
2. Premont, R.T. et al. (2000) J Biol Chem 275, 22373-80.
3. Frank, S.R. et al. (2006) EMBO J 25, 1848-59.
4. Brown, M.C. et al. (2005) Mol Biol Cell 16, 4316-28.
5. Yu, J.A. et al. (2009) Mol Biol Cell 20, 4706-19.
6. Yu, J.A. et al. (2010) Cell Adh Migr 4, 342-7.
7. Mazaki, Y. et al. (2006) Nat Immunol 7, 724-31.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phospho-GIT2 (Tyr392) (D8N9A) Rabbit mAb recognizes endogenous levels of GIT2 protein only when phosphorylated at Tyr392. This antibody may cross-react weakly with other tyrosine-phosphorylated proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr392 of human GIT2 protein.

Background

G protein-coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT1 and GIT2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac, and cdc42), kinases (such as PAK and MEK), the Rho family GEF Pix, and the focal adhesion protein paxillin (reviewed in 1). GIT1 and GIT2 share many of the same properties, but with at least ten distinct, tissue-specific splice variants. GIT2 has been shown to play an important role in inhibiting focal adhesion turnover and membrane protrusion (2,3). Focal adhesion localization and paxillin binding of GIT2 is regulated through phosphorylation at one or more tyrosine sites (Tyr286, Tyr392, Tyr592) by FAK and/or Src (4,5,reviewed in 6). Once at the focal adhesion, GIT2 is thought to play a key role in cell polarity and migration, making it a protein of interest in the investigation of oncogenic signaling pathways (3,5,7).

1. Hoefen, R.J. and Berk, B.C. (2006) J Cell Sci 119, 1469-75.
2. Premont, R.T. et al. (2000) J Biol Chem 275, 22373-80.
3. Frank, S.R. et al. (2006) EMBO J 25, 1848-59.
4. Brown, M.C. et al. (2005) Mol Biol Cell 16, 4316-28.
5. Yu, J.A. et al. (2009) Mol Biol Cell 20, 4706-19.
6. Yu, J.A. et al. (2010) Cell Adh Migr 4, 342-7.
7. Mazaki, Y. et al. (2006) Nat Immunol 7, 724-31.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.
Recommended Antibody Dilutions:
Western blotting 1:1000