PAX5 (D19F8) XP? Rabbit mAb (Alexa Fluor? 647 Conjugate)

• 产品编号：CST-11852S      品牌：Cell Signaling Technology       原厂货号：11852S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

PAX5 (D19F8) XP^® Rabbit mAb 兔单抗(Alexa Fluor^® 647 偶联)可以识别内源性的总PAX5蛋白。此单克隆抗体由合成肽段免疫动物产生，该肽段与人PAX5蛋白Gln350邻近氨基酸残基序列一致。此 Cell Signaling Technology公司抗体偶联Alexa Fluor^® 647 荧光染色，并经人类细胞免疫荧光分析进行内部测试。抗体预期和未偶联的PAX5 (D19F8) XP^® Rabbit mAb兔单抗 #8970显示同样的物种交叉反应。

1. Lang, D. et al. (2007) Biochem Pharmacol 73, 1-14.
2. Robson, E.J. et al. (2006) Nat Rev Cancer 6, 52-62.
3. Wang, Q. et al. (2008) J Cell Mol Med 12, 2281-94.
4. Blake, J.A. et al. (2008) Dev Dyn 237, 2791-803.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Xenopus

Specificity / Sensitivity

 PAX5 (D19F8) XP^® Rabbit mAb (Alexa Fluor^® 647 Conjugate) recognizes endogenous levels of total PAX5 protein.

Source / Purification

 Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln350 of human PAX5 protein.

Description

 This Cell Signaling Technology antibody is conjugated to Alexa Fluor^®647 fluorescent dye and tested in-house for immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated PAX5 (D19F8) XP^® Rabbit mAb #8970.

Background

 Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development (1). Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity (2). PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region (2). PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis (3); a subset of PAX proteins also maintain functional importance during postnatal development (4). Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes (1-3), with members of subgroups II and III identified as potential mediators of tumor progression (2).

1. Lang, D. et al. (2007) Biochem Pharmacol 73, 1-14.
2. Robson, E.J. et al. (2006) Nat Rev Cancer 6, 52-62.
3. Wang, Q. et al. (2008) J Cell Mol Med 12, 2281-94.
4. Blake, J.A. et al. (2008) Dev Dyn 237, 2791-803.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C

Recommended Antibody Dilutions:
Flow Cytometry 1:50