Akt (pan) (C67E7) Rabbit mAb (Magnetic Bead Conjugate)

• 产品编号：CST-11848S      品牌：CST       原厂货号：11848S
• 产品分类：抗体 > 一抗 > 其它类型一抗
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描述：

Akt (pan) (C67E7)Rabbit mAb（偶联磁珠）可以识别内源性总的Akt蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与小鼠Akt的羧基端邻近残基序列一致。Cell Signaling Technology抗体通过它的氨基酸结构与1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)激活的羧化物磁珠共价固定。Akt (pan) (C67E7)Rabbit mAb（偶联磁珠）可以用于免疫沉淀HA-偶联的蛋白。Akt，也被称为PKB或Rac，在控制存活和凋亡过程中发挥了关键作用（1-3）。该蛋白激酶可以被胰岛素和多种生长和存活因子激活，在设计PI3K激酶的wortmannin-sensitive信号通路中发挥作用（2,3）。与磷脂结合，和被PDK1磷酸化激活loop的Thr308以及磷酸化羧基端的Ser473可以激活Akt（4）。Akt的Ser473被磷酸化激发PDK2被认为是rapamycin-insensitive复合物和rictor以及Sin1中雷帕霉素的哺乳动物靶点(5,6).Akt通过磷酸化和失活某些靶点，例如Bad (7), forkhead transcription factors (8), c-Raf (9), 以及caspase-9，可以一直凋亡促进细胞存活。PTEN磷酸酶是PI3K 激酶/Akt信号通路的主要负调因子（10）。LY294002是一个特异性的PI3K激酶抑制剂（11）。Akt的另一个关键作用是通过磷酸化和失活GSK-3α 和 β调控糖原合成(12,13).Akt可能也在胰岛素刺激的糖原转位作用中发挥功能（12）。除了在存活和糖原合成过程中的作用，Akt还涉及细胞周期抑制GSK-3β接到的磷酸化和降解细胞周期D1（14）并且负调控细胞周期依赖的激酶抑制剂p27 Kip1 (15)和p21 Waf1/Cip1 (16)。Akt也直接调控含有raptor的rapamycin-sensitive复合物中mTOR的磷酸化，发挥调控细胞生长的作用（17）。更重要的是，Akt磷酸化并失活s tuberin (TSC2)，这是mTOR-raptor复合物中mTOR的抑制子（18,19）。

1. Franke, T.F. et al. (1997) Cell 88, 435-7.
2. Burgering, B.M. and Coffer, P.J. (1995) Nature 376, 599-602.
3. Franke, T.F. et al. (1995) Cell 81, 727-36.
4. Alessi, D.R. et al. (1996) EMBO J 15, 6541-51.
5. Sarbassov, D.D. et al. (2005) Science 307, 1098-101.
6. Jacinto, E. et al. (2006) Cell 127, 125-37.
7. Cardone, M.H. et al. (1998) Science 282, 1318-21.
8. Brunet, A. et al. (1999) Cell 96, 857-68.
9. Zimmermann, S. and Moelling, K. (1999) Science 286, 1741-4.
10. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
11. Vlahos, C.J. et al. (1994) J Biol Chem 269, 5241-8.
12. Hajduch, E. et al. (2001) FEBS Lett 492, 199-203.
13. Cross, D.A. et al. (1995) Nature 378, 785-9.
14. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.
15. Gesbert, F. et al. (2000) J Biol Chem 275, 39223-30.
16. Zhou, B.P. et al. (2001) Nat Cell Biol 3, 245-52.
17. Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.
18. Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57.
19. Manning, B.D. et al. (2002) Mol Cell 10, 151-62.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Pig

Specificity / Sensitivity

Akt (pan) (C67E7) Rabbit mAb (Magnetic Bead Conjugate) recognizes endogenous levels of total Akt protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues in the carboxy-terminal sequence of mouse Akt protein.

Description

This Cell Signaling Technology antibody is covalently immobilized to 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) activated carboxylated magnetic beads through its amino groups. Akt (pan) (C67E7) Rabbit mAb (Magnetic Bead Conjugate) is useful for immunoprecipitation assays of HA-tagged proteins.

Background

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip1 (15) and p21 Waf1/Cip1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).

1. Franke, T.F. et al. (1997) Cell 88, 435-7.
2. Burgering, B.M. and Coffer, P.J. (1995) Nature 376, 599-602.
3. Franke, T.F. et al. (1995) Cell 81, 727-36.
4. Alessi, D.R. et al. (1996) EMBO J 15, 6541-51.
5. Sarbassov, D.D. et al. (2005) Science 307, 1098-101.
6. Jacinto, E. et al. (2006) Cell 127, 125-37.
7. Cardone, M.H. et al. (1998) Science 282, 1318-21.
8. Brunet, A. et al. (1999) Cell 96, 857-68.
9. Zimmermann, S. and Moelling, K. (1999) Science 286, 1741-4.
10. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
11. Vlahos, C.J. et al. (1994) J Biol Chem 269, 5241-8.
12. Hajduch, E. et al. (2001) FEBS Lett 492, 199-203.
13. Cross, D.A. et al. (1995) Nature 378, 785-9.
14. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.
15. Gesbert, F. et al. (2000) J Biol Chem 275, 39223-30.
16. Zhou, B.P. et al. (2001) Nat Cell Biol 3, 245-52.
17. Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.
18. Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57.
19. Manning, B.D. et al. (2002) Mol Cell 10, 151-62.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Recommended Antibody Dilutions:
Immunoprecipitation 1:20