GST (26H1) Mouse mAb (Magnetic Bead Conjugate)

产品编号：CST-11847S 品牌：CST 原厂货号：11847S
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描述：

GST (26H1) Mouse mAb (Magnetic Bead Conjugate)能检测转染的GST融合蛋白。GST (26H1) Mouse mAb (Magnetic Bead Conjugate)能检测转染的GST融合蛋白。CST公司的抗体与甲酰基芳酰胺修饰的抗体交叉反应被固化，这种修饰的抗体带酰肼激活的磁珠。GST (26H1) Mouse mAb (Magnetic Bead Conjugate) 可用来做带有GST标签蛋白的免疫共沉淀。添加10μl 涡旋过的珠子至在1 x 1毫克/毫升1X Cell Lysis Buffer (10X) #9803中的200μl细胞裂解产物。更多细节请参见使用手册。为了免疫沉淀复合体的进一步洗脱和磁珠的清洗, 可用6-Tube Magnetic Separation Rack #7017分开磁珠。将包含磁珠的试管放置在磁力架上,等待1-2分钟，仔细去除上清。将试管重新取下,添加新的溶液，通过轻轻地涡旋与震荡试管重新悬浮磁珠。

附加表位对于用免疫印迹、免疫沉淀和免疫染色技术来进行蛋白质的标记和检测很有用。因为它们很小，不大可能会影响被标记蛋白的生化特性。

原厂资料：

Specificity / Sensitivity

GST (26H1) Mouse mAb (Magnetic Bead Conjugate) detects transfected glutathione S-transferase (GST) fusion proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a GST fusion protein.

Description

This Cell Signaling Technology antibody is immobilized by the covalent reaction of formylbenzamide-modified antibody with hydrazide-activated magnetic bead. GST (26H1) Mouse mAb (Magnetic Bead Conjugate) is useful for immunoprecipitation assays of GST-tagged proteins.

Directions for Use

Add 10 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details.

For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution using our 6-Tube Magnetic Separation Rack #7017. Place the tubes containing the beads in the Magnetic Separation Rack and wait 1 to 2 minutes for the solution to clear before carefully removing the supernatant. Remove the tubes from the Magnetic Separation Rack, add new solution and resuspend the beads by gently vortexing or rocking the tube.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

注意事项：

Storage: Supplied in PBS Buffer (pH 7.2), 0.1% Tween® 20. Store at 4°C. Do not aliquot the antibodies.

Directions for Use: Add 10 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details.

Recommended Antibody Dilutions: Immunoprecipitation 1:20