Phospho-Tau (Ser400/Thr403/Ser404) Antibody

• 产品编号：CST-11837S      品牌：CST       原厂货号：11837S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Tau (Ser400/Thr403/Ser404) Antibody兔多抗可识别内源性Ser400或Thr403或Ser404磷酸化的Tau蛋白。该抗体能够检测Ser400/Thr403，Ser400/Ser404或Thr403/Ser404的双位点磷酸化，也能检测Ser400/Thr403/Ser404三位点磷酸化。该多克隆抗体是由合成的人源tau蛋白Ser400/Thr403/Ser404肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。

Tau是微管相关蛋白异构物，尤其是在轴突中它能够促进和稳定微管装配。已鉴定Tau有六个亚型，其氨基末端有不同的的插入，羧基末端有不同数量的串联重复序列，tau蛋白可被ERK、GSK-3和CDK5在大约25个位点处被超磷酸化（1-2）。Tau蛋白磷酸化可降低其与微观的结合能力。神经纤维缠结是阿尔茨海默氏症的一个主要标志，这些缠结是由超磷酸化tau蛋白组成的成对螺旋细丝束。特别是在阿尔茨海默氏症中，GSK-3或CDK5对Tau蛋白Ser396的磷酸化可以导致微管的破坏。此外，Tau蛋白包涵体还在其它神经退行性疾病中被发现，这些疾病被统称为Tau蛋白病。研究已表明，阿尔茨海默氏病中，Tau在丝氨酸404位发生超磷酸化（4-7），这对微管有损害作用。

1. Johnson , G.V. and Stoothoff , W.H. (2004) J. Cell Sci. 117, 5721-5729.
2. Hanger, D. P. et al. (1998) J. Neurochem. 71, 2465-2476.
3. Bramblett, G. T. et al. (1993) Neuron 10, 1089-1099.
4. Shiurba, R.A. et al. (1996) Brain Res 737, 119-32.
5. Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
6. Evans, D.B. et al. (2000) J Biol Chem 275, 24977-83.
7. Bertrand, J. et al. (2010) Neuroscience 168, 323-34.

原厂资料：

Specificity / Sensitivity

Phospho-Tau (Ser400/Thr403/Ser404) Antibody recognizes endogenous levels of tau protein when phosphorylated at Ser400 or Thr403 or Ser404. This antibody also detects dual phosphorylation at Ser400/Thr403, Ser400/Ser404, or Thr403/Ser404, and triple phosphorylation at Ser400/Thr403/Ser404.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser400/Thr403/Ser404 of human tau protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, GSK-3, and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3). Investigators have shown that tau phosphorylation at Ser404 destabilizes microtubules and that tau is hyperphosphorylated at Ser404 in Alzheimer's disease (4-7).

1. Johnson , G.V. and Stoothoff , W.H. (2004) J. Cell Sci. 117, 5721-5729.
2. Hanger, D. P. et al. (1998) J. Neurochem. 71, 2465-2476.
3. Bramblett, G. T. et al. (1993) Neuron 10, 1089-1099.
4. Shiurba, R.A. et al. (1996) Brain Res 737, 119-32.
5. Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
6. Evans, D.B. et al. (2000) J Biol Chem 275, 24977-83.
7. Bertrand, J. et al. (2010) Neuroscience 168, 323-34.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM  NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.  Do not aliquot the antibody.

*Species cross-reactivity is determined by western blot.

** Anti-rabbit secondary antibodies must be used to  detect this antibody.

Recommended Antibody Dilutions:

Western blotting 1:1000