WBP2 Antibody

• 产品编号：CST-11831S      品牌：CST       原厂货号：11831S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

WBP2抗体可以识别内源性的总WBP2蛋白。多抗由合成肽段免疫动物产生，该肽段与人WBP2蛋白Asn239邻近氨基酸残基序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。多抗由合成肽段免疫动物产生，该肽段与人WBP2蛋白Asn239邻近氨基酸残基序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。

1. Chen, H.I. and Sudol, M. (1995) Proc Natl Acad Sci U S A 92, 7819-23.
2. Chen, H.I. et al. (1997) J Biol Chem 272, 17070-7.
3. McDonald, C.B. et al. (2011) Biochemistry 50, 9616-27.
4. Chan, S.W. et al. (2011) Oncogene 30, 600-10.
5. Jolliffe, C.N. et al. (2000) Biochem J 351 Pt 3, 557-65.
6. Zhang, X. et al. (2011) Cell Death Differ 18, 1346-55.
7. Dhananjayan, S.C. et al. (2006) Mol Endocrinol 20, 2343-54.
8. Lim, S.K. et al. (2011) FASEB J 25, 3004-18.

原厂资料：

Specificity / Sensitivity

WBP2 Antibody recognizes endogenous levels of total WBP2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn239 of human WBP2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

WW-domain binding protein 2 (WBP2) is an adaptor protein first identified in a screen for proteins that interact with YAP1 (1). WBP2 was subsequently shown to bind other WW domain-containing proteins, including TAZ and NEDD4-like ubiquitin-protein ligases (2-5). There is strong evidence for a conserved functional role for WBP2 in the Hippo kinase tumor suppressor pathway. WBP2 interaction is required for the oncogenic properties of TAZ in breast cancer cells (4) and the growth-promoting properties of the YAP homolog Yorkie in Drosophila (6). In vitro studies have also suggested that WBP2 may act as a transcriptional co-activator of both estrogen and progesterone receptors (7); this interaction was shown to be dependent upon tyrosine phosphorylation of WBP2 by c-Src and c-Yes kinases (8).

1. Chen, H.I. and Sudol, M. (1995) Proc Natl Acad Sci U S A 92, 7819-23.
2. Chen, H.I. et al. (1997) J Biol Chem 272, 17070-7.
3. McDonald, C.B. et al. (2011) Biochemistry 50, 9616-27.
4. Chan, S.W. et al. (2011) Oncogene 30, 600-10.
5. Jolliffe, C.N. et al. (2000) Biochem J 351 Pt 3, 557-65.
6. Zhang, X. et al. (2011) Cell Death Differ 18, 1346-55.
7. Dhananjayan, S.C. et al. (2006) Mol Endocrinol 20, 2343-54.
8. Lim, S.K. et al. (2011) FASEB J 25, 3004-18.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to detect this antibody.
Recommended Antibody Dilutions:
Western blotting 1:1000