FKBP4 Antibody

• 产品编号：CST-11826S      品牌：CST       原厂货号：11826S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

FKBP4 Antibody 能够检测内源性的FKBP4总蛋白。该抗体不会与其他FKBP4蛋白发生交叉反应。该多克隆抗体是经由合成的围绕人FKBP4蛋白Met440位点的氨基酸肽段免疫动物而生产的。经过Protein A和亲和层析纯化。FKBP4（也称FKBP52）是亲免蛋白家族的成员之一。尽管FKBP4能够与免疫抑制剂FK506和rapamycin结合，但并没有显示明显的该家族典型具有的免疫抑制活性 。（1,2）。FKBP4对于B和T淋巴细胞中的免疫调控基因表达具有重要作用，但其在调控甾体激素受体信号通路以及细胞骨架动力学中的作用也有重大意义。FKBP4含有两个肽酰-脯氨酰-顺反式异构酶 (PPIase)结构域，第一个参与了甾体激素受体信号通路，而第二个能够与tubulin以及其他细胞骨架组分相互作用。胞质内甾体激素受体成熟为一个功能性的构象需要多种分子伴侣和共分子伴侣组分，包括HSP90, p23, 以及FKBP4 (3,4)。FKBP4与HSP90相互作用可以促进雄性激素, 糖皮质激素 以及黄体酮甾体激素受体的折叠。实际上，在FKBP4缺失的情况下，这些受体的功能性被破坏。而且在敲除的动物里的研究表明老鼠会呈现雄激素不敏感综合征的特征（5）。另外，在脑中高水平表达的FKBP4 可与高度磷酸化的Tau相互作用并且对抗Tau的促进微管聚合的作用（6）。FKBP4还能够通过将APP (阿尔茨海默淀粉样前体蛋白)加工成为伸展聚合物来抑制果蝇中β淀粉样蛋白毒性（7）。

1. Peattie, D.A. et al. (1992) Proc Natl Acad Sci U S A 89, 10974-8.
2. Wu, B. et al. (2004) Proc Natl Acad Sci U S A 101, 8348-53.
3. De Leon, J.T. et al. (2011) Proc Natl Acad Sci U S A 108, 11878-83.
4. Ebong, I.O. et al. (2011) Proc Natl Acad Sci U S A 108, 17939-44.
5. Sivils, J.C. et al. (2011) Curr Opin Pharmacol 11, 314-9.
6. Chambraud, B. et al. (2010) Proc Natl Acad Sci U S A 107, 2658-63.
7. Sanokawa-Akakura, R. et al. (2010) PLoS One 5, e8626.

原厂资料：

Specificity / Sensitivity

FKBP4 Antibody recognizes endogenous levels of total FKBP4 protein. This antibody does not cross-react with other FKBP proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Met440 of human FKBP4 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

FKBP4 (also known as FKBP52) is a member of the immunophilin protein family. FKBP4 does not demonstrate appreciable immunosuppressant activity typical of this family, despite its ability to bind the immunosuppressants FK506 and rapamycin (1,2). While FKBP4 plays an important role in immunoregulatory gene expression in B and T lymphocytes, its role in regulating steroid hormone receptor signaling and cytoskeletal dynamics is garnering significant interest. FKBP4 contains two petidyl-prolyl cis-trans isomerase (PPIase) domains, the first of which is implicated in steroid receptor signaling while the second interacts with tubulin and other cytoskeletal components. The maturation of cytoplasmic steroid hormone receptors into a functional conformation requires multiple chaperone and co-chaperone components, including HSP90, p23, and FKBP4 (3,4). FKBP4 interacts with HSP90 to facilitate the folding of androgen, glucocorticoid, and progesterone steroid hormone receptors. Indeed, the functionality of these receptors is impaired in the absence of FKBP4, and research studies have found that null mice demonstrate signs of androgen insensitivity syndrome (5). In addition, FKBP4, which is expressed at high levels in the brain, interacts with hyperphosphorylated Tau and antagonizes Tau's ability to promote microtubule polymerization (6). FKBP4 can also suppress amyloid β toxicity in Drosophila by processing APP (Alzheimer's Amyloid Precursor Protein) to unfold aggregates (7).

1. Peattie, D.A. et al. (1992) Proc Natl Acad Sci U S A 89, 10974-8.
2. Wu, B. et al. (2004) Proc Natl Acad Sci U S A 101, 8348-53.
3. De Leon, J.T. et al. (2011) Proc Natl Acad Sci U S A 108, 11878-83.
4. Ebong, I.O. et al. (2011) Proc Natl Acad Sci U S A 108, 17939-44.
5. Sivils, J.C. et al. (2011) Curr Opin Pharmacol 11, 314-9.
6. Chambraud, B. et al. (2010) Proc Natl Acad Sci U S A 107, 2658-63.
7. Sanokawa-Akakura, R. et al. (2010) PLoS One 5, e8626.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to detect this antibody.
Recommended Antibody Dilutions:
Western blotting 1:1000