Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb

• 产品编号：CST-11818S      品牌：CST       原厂货号：11818S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb兔单抗能够检测内源性的在79位丝氨酸磷酸化的乙酰辅酶A羧化酶蛋白水平。该单克隆抗体是由合成的人源酰辅酶A羧化酶蛋白丝氨酸(79位)的肽段免疫动物而制备的。

乙酰辅酶A羧化酶（ACC）催化乙酰辅酶A羧化形成丙二酰辅酶A（1）。这是脂肪酸生物合成和氧化过程中的关键酶（1）。在啮齿类动物中，脂肪组织中主要表达的是265 kDa的ACC1（ACCα），而280 kDa的ACC2（ACCβ）是氧化组织中的主要构型（1,2）。然而，在人体中，ACC2在脂肪与氧化组织均是主要构型（1,2）。AMPK催化的Ser79磷酸化与PKA催化的 Ser1200磷酸化会抑制ACC的酶活性（3）。ACC是抗肥胖药物的潜在靶点（4,5）。

1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.

原厂资料：

Specificity / Sensitivity

Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb recognizes endogenous levels of acetyl-CoA carboxylase protein only when phosphorylated at Ser79.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser79 of human acetyl-CoA carboxylase protein.

Background

Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5)。

1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to detect this antibody.
Recommended Antibody Dilutions:
Western blotting 1:1000
Immunoprecipitation 1:50
Immunohistochemistry (Paraffin) 1:400†
 Unmasking buffer: Citrate
 Antibody diluent: SignalStain® Antibody Diluent #8112
 Detection reagent: SignalStain® Boost (HRP, Rabbit) #8114
†Optimal IHC dilutions determined using SignalStain® Boost IHC Detection Reagent.
Immunofluorescence (IF-IC) 1:250